Human ILK-1 (Integrin-Linked Kinase 1) ELISA Kit | ILK-1 elisa kit

Human ILK-1 (Integrin-Linked Kinase 1) ELISA Kit

Cat. Num. AAA7606706

• Gene Names: ILK; P59; ILK-1; ILK-2; p59ILK; HEL-S-28
• Reactivity: Human
• Synonyms: ILK-1 (Integrin-Linked Kinase 1); Human ILK-1 (Integrin-Linked Kinase 1) ELISA Kit; ILK-1 elisa kit

• Reactivity: Human
• Sequence Length: 452

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 78.125-5000pg/ml
• Sensitivity: 46.875pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ILK-1 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- ILK-1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-ILK-1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the ILK-1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of ILK-1 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 9973397
• NCBI GeneID: 3611
• UniProt Accession #: Q13418; P57043; Q68DZ3; B7Z1I0; B7Z418; D3DQU0
• Molecular Weight: 36,467 Da
• NCBI Official Full Name: Integrin-linked protein kinase
• NCBI Official Synonym Full Names: integrin linked kinase
• NCBI Official Symbol: ILK
• NCBI Official Synonym Symbols: P59; ILK-1; ILK-2; p59ILK; HEL-S-28
• NCBI Protein Information: integrin-linked protein kinase
• UniProt Protein Name: Integrin-linked protein kinase
• UniProt Gene Name: ILK
• UniProt Synonym Gene Names: ILK1; ILK2

NCBI Description

This gene encodes a protein with a kinase-like domain and four ankyrin-like repeats. The encoded protein associates at the cell membrane with the cytoplasmic domain of beta integrins, where it regulates integrin-mediated signal transduction. Activity of this protein is important in the epithelial to mesenchymal transition, and over-expression of this gene is implicated in tumor growth and metastasis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]

Uniprot Description

ILK: a tyrosine kinase-like kinase of the MLK family. Couples integrins and growth factors to downstream pathways involved in cell survival, cell cycle control, cell-cell adhesion and cell motility. Functions as a scaffold bridging the extra-cellular matrix and growth factor receptors to the actin cytoskeleton through interactions with the ILK-PINCH complex. This complex, which includes integrin, PINCH (which links ILK to receptor tyrosine kinases via Nck2), PARVA and affixin, is considered to be one of the convergence points of integrin- and growth factor-signaling pathways. May be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Stimulated downstream of PI3 kinase. Increased expression is correlated with progression of several tumor types, including breast, prostate, and colon carcinomas. Overexpression drives anchorage-independent growth and faster cell cycle.

Protein type: EC 2.7.11.1; ILK subfamily; Kinase, protein; MLK family; Protein kinase, Ser/Thr (non-receptor); Protein kinase, TKL; TKL group

Chromosomal Location of Human Ortholog: 11p15.4

Cellular Component: cell junction; cytoplasm; cytosol; focal adhesion; membrane; nucleoplasm; stress fiber

Molecular Function: protein binding; protein kinase binding; protein serine/threonine kinase activity; signal transducer activity

Biological Process: cell-matrix adhesion; integrin-mediated signaling pathway; MAPKKK cascade; multicellular organismal development; positive regulation of phosphorylation; positive regulation of transcription, DNA-dependent; protein amino acid phosphorylation; tumor necrosis factor-mediated signaling pathway