IL-32 elisa kit

Human IL-32 PicoKine ELISA Kit

Cat. Num. AAA1751560

• Gene Names: IL32; NK4; TAIF; TAIFa; TAIFb; TAIFc; TAIFd; IL-32beta; IL-32alpha; IL-32delta; IL-32gamma
• Reactivity: Human; There is no detectable cross-reactivity with other relevant proteins.
• Synonyms: IL-32; Human IL-32 PicoKine ELISA Kit; Human IL-32; Interleukin-32; Natural killer cells protein 4; Tumor necrosis factor alpha-inducing factor; IL32; NK4; TAIF; Interleukin 32; IL-32 elisa kit

• Reactivity: Human; There is no detectable cross-reactivity with other relevant proteins.
• Specificity: Natural and recombinant human IL-32
• Sequence Length: 188

• Samples: Cell Culture Supernates, Cell Lysates, Serum And Plasma (Heparin, Edta)
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision
• Inter-assay Precision: Inter-Assay Precision (Precision accross assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months. Store at -20 degree C for 12 months.; Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IL-32 elisa kit

Principle of the Assay: The Picokine Human IL22 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human IL22 with a 96-well strip plate that is pre-coated with antibody specific for IL22. The detection antibody is a biotinylated antibody specific for IL22. The capture antibody is monoclonal antibody from mouse, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Human IL22 with immunogen: Expression system for standard: E.coli; Immunogen sequence: A34-I179. The kit is analytically validated with ready to use reagents. To measure Human IL22, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly propotional to Human IL22 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human IL22 in the sample!!Background/Introduction: Interleukin-22(IL-22), also known as ILTIF, is protein that in humans is encoded by the IL22 gene. IL-22 a member of a group of cytokines called the IL-10 family or IL-10 superfamily a class of potent mediators of cellular inflammatory responses. Using FISH, the IL22 gene is mapped to chromosome 12q15, close to the IFNG and the herpesvirus saimiri-induced AK155 genes. IL-22 can contribute to immune disease through the stimulation of inflammatory responses, S100s and defensins. IL-22 also promotes hepatocyte survival in the liver and epithelial cells in the lung and gut similar to IL-10. In some contexts, the pro-inflammatory versus tissue-protective functions of IL-22 are regulated by the often coexpressed cytokine IL-17A.

NCBI and Uniprot Product Information

• NCBI GI #: 61639466
• NCBI GeneID: 9235
• NCBI Accession #: NP_001012649.1
• NCBI GenBank Nucleotide #: NM_001012631.1
• UniProt Accession #: P24001; Q5VFH7; Q5VFH8; Q8WV38; Q96GK9; A6NNM0; A8MPX0; B4DJM1; B8Q191; D3DUB0; D3DUB2
• Molecular Weight: 21,737 Da
• NCBI Official Full Name: interleukin-32 isoform B
• NCBI Official Synonym Full Names: interleukin 32
• NCBI Official Symbol: IL32
• NCBI Official Synonym Symbols: NK4; TAIF; TAIFa; TAIFb; TAIFc; TAIFd; IL-32beta; IL-32alpha; IL-32delta; IL-32gamma
• NCBI Protein Information: interleukin-32
• UniProt Protein Name: Interleukin-32
• UniProt Gene Name: IL32
• UniProt Synonym Gene Names: NK4; TAIF; IL-32

NCBI Description

This gene encodes a member of the cytokine family. The protein contains a tyrosine sulfation site, 3 potential N-myristoylation sites, multiple putative phosphorylation sites, and an RGD cell-attachment sequence. Expression of this protein is increased after the activation of T-cells by mitogens or the activation of NK cells by IL-2. This protein induces the production of TNFalpha from macrophage cells. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

Cytokine that may play a role in innate and adaptive immune responses. It induces various cytokines such as TNFA/TNF-alpha and IL8. It activates typical cytokine signal pathways of NF-kappa-B and p38 MAPK.