Human SH3 Domain Binding Protein 2 (SH3BP2) ELISA Kit | SH3BP2 elisa kit

Human SH3 Domain Binding Protein 2 (SH3BP2) ELISA Kit

Cat. Num. AAA456152

• Gene Names: SH3BP2; 3BP2; CRBM; CRPM; 3BP-2; RES4-23
• Reactivity: Human
• Synonyms: SH3 Domain Binding Protein 2 (SH3BP2); Human SH3 Domain Binding Protein 2 (SH3BP2) ELISA Kit; SH3BP2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of SH3BP2. No significant cross-reactivity or interference between SH3BP2 and analogues was observed.
• Sequence Length: 618

• Samples: Tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.312-20ng/mL
• Sensitivity: 0.126ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level SH3BP2 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level SH3BP2 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SH3BP2 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of SH3BP2 in human tissue homogenates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to SH3BP2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to SH3BP2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain SH3BP2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of SH3BP2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 170014705
• NCBI GeneID: 6452
• NCBI Accession #: NP_001116153.1
• NCBI GenBank Nucleotide #: NM_001122681.1
• UniProt Accession #: P78314; O00500; O15373; P78315; A6NNC2; B2R5R6; B4DT04; D3DVR0; D6R919
• Molecular Weight: 68,129 Da
• NCBI Official Full Name: SH3 domain-binding protein 2 isoform a
• NCBI Official Synonym Full Names: SH3 domain binding protein 2
• NCBI Official Symbol: SH3BP2
• NCBI Official Synonym Symbols: 3BP2; CRBM; CRPM; 3BP-2; RES4-23
• NCBI Protein Information: SH3 domain-binding protein 2
• UniProt Protein Name: SH3 domain-binding protein 2
• Protein Family: SH3 domain-binding protein
• UniProt Gene Name: SH3BP2
• UniProt Synonym Gene Names: 3BP2; 3BP-2

NCBI Description

The protein encoded by this gene has an N-terminal pleckstrin homology (PH) domain, an SH3-binding proline-rich region, and a C-terminal SH2 domain. The protein binds to the SH3 domains of several proteins including the ABL1 and SYK protein tyrosine kinases , and functions as a cytoplasmic adaptor protein to positively regulate transcriptional activity in T, natural killer (NK), and basophilic cells. Mutations in this gene result in cherubism. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]

Uniprot Description

Binds differentially to the SH3 domains of certain proteins of signal transduction pathways. Binds to phosphatidylinositols; linking the hemopoietic tyrosine kinase fes to the cytoplasmic membrane in a phosphorylation dependent mechanism.