Human IGFBP6 ELISA Kit | IGFBP6 elisa kit

Human IGFBP6 PicoKine ELISA Kit

Cat. Num. AAA177302

• Gene Names: IGFBP6; IBP6
• Reactivity: Human
• Synonyms: IGFBP6; Human IGFBP6 PicoKine ELISA Kit; Insulin-like growth factor-binding protein 6; insulin-like growth factor binding protein 6; IBP 6; IBP-6; IBP6; IBP6_HUMAN; IGF binding protein 6; IGF-binding protein 6; IGFBP 6; IGFBP-6; Insulin like growth factor binding protein 6; IGFBP6 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant Human IGFBP6
• Sequence Length: 240

• Samples: Cell culture supernatants, cell lysates, serum and plasma (heparin, edta)
• Detection Range: 62.5 pg/ml - 4,000 pg/ml
• Sensitivity: <10 pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision across assays): Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IGFBP6 elisa kit

Principle of the Assay: The Picokine™ Human IGFBP6 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Human IGFBP6 with a 96-well strip plate that is pre-coated with antibody specific for IGFBP6. The detection antibody is a biotinylated antibody specific for IGFBP6. The capture antibody is monoclonal antibody from mouse and the detection antibody is polyclonal antibody from goat. The kit contains recombinant Human IGFBP6 with immunogen: Expression system for standard: NS0; Immunogen sequence: A25-G240. The kit is analytically validated with ready-to-use reagents. To measure Human IGFBP6, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Human IGFBP6 in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human IGFBP6 in the sample.

Background/Introduction: Insulin-like growth factor-binding protein 6 (IGFBP6) is a protein that in humans is encoded by the IGFBP6 gene. The IGFBP6 gene is mapped to chromosome 12. In all, it has contained 216 amino acid protein with a calculated molecular mass of 22,847, The human keratinocyte cell line HaCat secretes IGFBP6 as an autocrine growth inhibitor. Recombinant IGFBP6 was also shown to inhibit growth of HaCat cells and other keratinocyte cell lines.

NCBI and Uniprot Product Information

• NCBI GI #: 124068
• NCBI GeneID: 3489
• UniProt Accession #: P24592; Q14492
• Molecular Weight: 25,322 Da
• NCBI Official Full Name: Insulin-like growth factor-binding protein 6
• NCBI Official Synonym Full Names: insulin-like growth factor binding protein 6
• NCBI Official Symbol: IGFBP6
• NCBI Official Synonym Symbols: IBP6
• NCBI Protein Information: insulin-like growth factor-binding protein 6; IBP-6; IGFBP-6; IGF binding protein 6; IGF-binding protein 6
• UniProt Protein Name: Insulin-like growth factor-binding protein 6
• Protein Family: Insulin-like growth factor-binding protein
• UniProt Gene Name: IGFBP6
• UniProt Synonym Gene Names: IBP6; IBP-6; IGF-binding protein 6; IGFBP-6
• UniProt Entry Name: IBP6_HUMAN

Uniprot Description

IGFBP6: IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.

Protein type: Cell cycle regulation; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: Golgi apparatus; extracellular space; extracellular region

Molecular Function: insulin-like growth factor I binding; insulin-like growth factor II binding

Biological Process: negative regulation of cell proliferation; regulation of insulin-like growth factor receptor signaling pathway; cellular protein metabolic process; regulation of cell growth; signal transduction