Monkey Inducible T-cell Costimulator ELISA Kit | CD278/ICOS elisa kit

Monkey Inducible T-cell Costimulator ELISA Kit

Cat. Num. AAA750627

• Gene Names: ICOS; AILIM; CD278; CVID1
• Reactivity: Monkey
• Synonyms: Inducible T-cell Costimulator; Monkey Inducible T-cell Costimulator ELISA Kit; CD278/ICOS elisa kit

• Reactivity: Monkey

• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve

Related Product Information for CD278/ICOS elisa kit

Intended Uses: This ICOS ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey ICOS. This ELISA kit for research use only!

Principle of the Assay||ICOS ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ICOS antibody and an ICOS-HRP conjugate. The assay sample and buffer are incubated together with ICOS-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ICOS concentration since ICOS from samples and ICOS-HRP conjugate compete for the anti-ICOS antibody binding site. Since the number of sites is limited, as more sites are occupied by ICOS from the sample, fewer sites are left to bind ICOS-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ICOS concentration in each sample is interpolated from this standard curve.

Product Categories/Family for CD278/ICOS elisa kit

Immunology

NCBI and Uniprot Product Information

• NCBI GI #: 15029518
• NCBI GeneID: 29851
• NCBI Accession #: NP_036224.1
• NCBI GenBank Nucleotide #: NM_012092.3
• Molecular Weight: 19,127 Da
• NCBI Official Full Name: inducible T-cell costimulator
• NCBI Official Synonym Full Names: inducible T-cell co-stimulator
• NCBI Official Symbol: ICOS
• NCBI Official Synonym Symbols: AILIM; CD278; CVID1
• NCBI Protein Information: inducible T-cell costimulator; inducible costimulator; activation-inducible lymphocyte immunomediatory molecule
• UniProt Protein Name: Inducible T-cell costimulator
• Protein Family: Inducible T-cell costimulator
• UniProt Gene Name: ICOS
• UniProt Synonym Gene Names: AILIM
• UniProt Entry Name: ICOS_HUMAN

NCBI Description

The protein encoded by this gene belongs to the CD28 and CTLA-4 cell-surface receptor family. It forms homodimers and plays an important role in cell-cell signaling, immune responses, and regulation of cell proliferation. [provided by RefSeq, Jul 2008]

Uniprot Description

ICOS: Enhances all basic T-cell responses to a foreign antigen, namely proliferation, secretion of lymphokines, up- regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B-cells. Essential both for efficient interaction between T and B-cells and for normal antibody responses to T-cell dependent antigens. Does not up- regulate the production of interleukin-2, but superinduces the synthesis of interleukin-10. Prevents the apoptosis of pre- activated T-cells. Plays a critical role in CD40-mediated class switching of immunoglobin isotypes. Defects in ICOS are the cause of immunodeficiency common variable type 1 (CVID1). CVID1 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B-cells is usually in the normal range, but can be lo. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 2q33

Cellular Component: integral to plasma membrane; plasma membrane; extracellular region; external side of plasma membrane

Biological Process: cell-cell adhesion; T cell tolerance induction; T cell costimulation; immune response

Disease: Immunodeficiency, Common Variable, 2; Immunodeficiency, Common Variable, 1