Camel IFN-gamma (Interferon Gamma) ELISA Kit | IFN-gamma elisa kit

Camel IFN-gamma (Interferon Gamma) ELISA Kit

Cat. Num. AAA7606527

• Gene Names: IFNG; IFG; IFI
• Reactivity: Camel
• Synonyms: IFN-gamma (Interferon Gamma); Camel IFN-gamma (Interferon Gamma) ELISA Kit; Interferon Gamma; IFN-gamma elisa kit

• Reactivity: Camel
• Specificity: This assay has high sensitivity and excellent specificity for detection of IFN-gamma. No significant cross-reactivity or interference between IFN-gamma and analogues was observed.
• Sequence Length: 166

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 31.25-2000pg/ml
• Sensitivity: 18.75pg/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IFN-gamma elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 56786138
• NCBI GeneID: 3458
• NCBI Accession #: NP_000610.2
• NCBI GenBank Nucleotide #: NM_000619.2
• UniProt Accession #: P01579; Q53ZV4; B5BU88
• Molecular Weight: 19,348 Da
• NCBI Official Full Name: interferon gamma
• NCBI Official Synonym Full Names: interferon gamma
• NCBI Official Symbol: IFNG
• NCBI Official Synonym Symbols: IFG; IFI
• NCBI Protein Information: interferon gamma
• UniProt Protein Name: Interferon gamma
• UniProt Gene Name: IFNG
• UniProt Synonym Gene Names: IFN-gamma

NCBI Description

This gene encodes a soluble cytokine that is a member of the type II interferon class. The encoded protein is secreted by cells of both the innate and adaptive immune systems. The active protein is a homodimer that binds to the interferon gamma receptor which triggers a cellular response to viral and microbial infections. Mutations in this gene are associated with an increased susceptibility to viral, bacterial and parasitic infections and to several autoimmune diseases. [provided by RefSeq, Dec 2015]

Uniprot Description

IFNG: Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. Homodimer. Released primarily from activated T lymphocytes. Belongs to the type II (or gamma) interferon family.

Protein type: Cytokine; Membrane protein, integral; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 12q15

Cellular Component: extracellular region

Biological Process: apoptosis; cell cycle arrest; cell motility; cell surface receptor linked signal transduction; negative regulation of epithelial cell differentiation; negative regulation of interleukin-17 production; negative regulation of smooth muscle cell proliferation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; positive regulation of autophagy; positive regulation of CD4-positive, CD25-positive, alpha-beta regulatory T cell differentiation during immune response; positive regulation of cell proliferation; positive regulation of interleukin-12 production; positive regulation of interleukin-23 production; positive regulation of killing of cells of another organism; positive regulation of membrane protein ectodomain proteolysis; positive regulation of nitric oxide biosynthetic process; positive regulation of osteoclast differentiation; positive regulation of peptidyl-serine phosphorylation of STAT protein; positive regulation of protein amino acid phosphorylation; positive regulation of protein complex assembly; positive regulation of protein import into nucleus, translocation; protein import into nucleus, translocation; regulation of insulin secretion; response to virus

Disease: Aplastic Anemia; Hepatitis C Virus, Susceptibility To; Human Immunodeficiency Virus Type 1, Susceptibility To; Mycobacterium Tuberculosis, Susceptibility To; Tuberous Sclerosis 2