Human Homeobox protein OTX2 ELISA Kit | OTX2 elisa kit

Human Homeobox protein OTX2 ELISA Kit

Cat. Num. AAA2883898

• Gene Names: OTX2; CPHD6; MCOPS5
• Reactivity: Human
• Synonyms: Homeobox protein OTX2; Human Homeobox protein OTX2 ELISA Kit; Orthodenticle homolog 2; OTX2; OTX2 elisa kit

• Reactivity: Human
• Sequence Length: 289

• Samples: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates Or Other Biological Fluids.
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for OTX2 elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 395455071
• NCBI GeneID: 5015
• NCBI Accession #: NP_001257452.1
• NCBI GenBank Nucleotide #: NM_001270523.1
• UniProt Accession #: P32243; Q6GTV3; Q9HAW3; Q9P2R1; B2RAN5
• Molecular Weight: 32,406 Da
• NCBI Official Full Name: homeobox protein OTX2 isoform b
• NCBI Official Synonym Full Names: orthodenticle homeobox 2
• NCBI Official Symbol: OTX2
• NCBI Official Synonym Symbols: CPHD6; MCOPS5
• NCBI Protein Information: homeobox protein OTX2
• UniProt Protein Name: Homeobox protein OTX2
• Protein Family: Homeobox protein
• UniProt Gene Name: OTX2
• UniProt Entry Name: OTX2_HUMAN

NCBI Description

This gene encodes a member of the bicoid subfamily of homeodomain-containing transcription factors. The encoded protein acts as a transcription factor and plays a role in brain, craniofacial, and sensory organ development. The encoded protein also influences the proliferation and differentiation of dopaminergic neuronal progenitor cells during mitosis. Mutations in this gene cause syndromic microphthalmia 5 (MCOPS5) and combined pituitary hormone deficiency 6 (CPHD6). This gene is also suspected of having an oncogenic role in medulloblastoma. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Pseudogenes of this gene are known to exist on chromosomes two and nine. [provided by RefSeq, Jul 2012]

Uniprot Description

OTX2: Probably plays a role in the development of the brain and the sense organs. Can bind to the BCD target sequence (BTS): 5'-TCTAATCCC-3'. Expressed in brain. Belongs to the paired homeobox family. Bicoid subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 14q22.3

Cellular Component: cytoplasm; growth cone; nucleus; protein complex

Molecular Function: eukaryotic initiation factor 4E binding; protein binding; protein heterodimerization activity

Biological Process: axon guidance; cell fate specification; diencephalon morphogenesis; dorsal/ventral pattern formation; endoderm development; eye photoreceptor cell fate commitment; forebrain development; inner ear morphogenesis; metencephalon development; midbrain development; neuron fate determination; positive regulation of embryonic development; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; protein complex assembly; regulation of fibroblast growth factor receptor signaling pathway; regulation of smoothened signaling pathway; somite rostral/caudal axis specification; transcription from RNA polymerase II promoter

Disease: Microphthalmia, Syndromic 5; Pituitary Hormone Deficiency, Combined, 6