Human Histone Deacetylase 5 ELISA Kit | HDAC5 elisa kit

Human Histone Deacetylase 5 ELISA Kit

Cat. Num. AAA3804292

• Gene Names: HDAC5; HD5; NY-CO-9
• Reactivity: Human
• Synonyms: Histone Deacetylase 5; Human Histone Deacetylase 5 ELISA Kit; HDAC5 elisa kit

• Reactivity: Human
• Sequence Length: 1122

• Samples: Serum, plasma, cell culture supernate and other biological fluids
• Assay Type: Sandwich
• Detection Range: 125 pg/ml-2000 pg/ml
• Sensitivity: 10 pg/ml

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HDAC5 elisa kit

Intended Use: The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration ofHISTONE DEACETYLASE 5in the sample, This HISTONE DEACETYLASE 5ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versusHISTONE DEACETYLASE 5concentration. The concentration ofHISTONE DEACETYLASE 5in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 62750347
• NCBI GeneID: 10014
• NCBI Accession #: NP_005465.2
• NCBI GenBank Nucleotide #: NP_005465.2
• UniProt Accession #: Q9UQL6
• NCBI Official Full Name: histone deacetylase 5 isoform 1
• NCBI Official Synonym Full Names: histone deacetylase 5
• NCBI Official Symbol: HDAC5
• NCBI Official Synonym Symbols: HD5; NY-CO-9
• NCBI Protein Information: histone deacetylase 5
• UniProt Protein Name: Histone deacetylase 5
• Protein Family: Histone deacetylase
• UniProt Gene Name: HDAC5
• UniProt Synonym Gene Names: KIAA0600; HD5
• UniProt Entry Name: HDAC5_HUMAN

NCBI Description

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the class II histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. It coimmunoprecipitates only with HDAC3 family member and might form multicomplex proteins. It also interacts with myocyte enhancer factor-2 (MEF2) proteins, resulting in repression of MEF2-dependent genes. This gene is thought to be associated with colon cancer. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

HDAC5: a transcriptional regulator of the histone deacetylase family, subfamily 2. Deacetylates lysine residues on the N-terminal part of the core histones H2A, H2B, H3 AND H4. Plays an important role in transcriptional regulation, cell cycle progression and developmental events. Coimmunoprecipitates only with HDAC3 family members. Interacts with myocyte enhancer factor-2 (MEF2) proteins, resulting in repression of MEF2-dependent genes. Two alternatively spliced isoforms have been described.

Protein type: EC 3.5.1.98; Hydrolase

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: nucleoplasm; nuclear body; Golgi apparatus; histone deacetylase complex; cytoplasm; nucleus; cytosol

Molecular Function: NAD-dependent histone deacetylase activity (H3-K9 specific); protein binding; protein kinase C binding; NAD-dependent histone deacetylase activity (H3-K14 specific); histone deacetylase binding; metal ion binding; protein deacetylase activity; NAD-dependent histone deacetylase activity (H4-K16 specific); histone deacetylase activity; transcription corepressor activity; transcription factor binding

Biological Process: establishment and/or maintenance of chromatin architecture; heart development; chromatin silencing; negative regulation of transcription from RNA polymerase II promoter; regulation of gene expression, epigenetic; protein amino acid deacetylation; negative regulation of osteoblast differentiation; inflammatory response; response to drug; Notch signaling pathway; regulation of skeletal muscle fiber development; transcription, DNA-dependent; B cell activation; histone deacetylation; chromatin modification; response to cocaine; osteoblast development; chromatin remodeling; cellular response to insulin stimulus; regulation of protein binding; B cell differentiation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription factor activity; negative regulation of transcription, DNA-dependent; multicellular organismal response to stress