Immunohistochemistry (IHC)
(Anti-CD69 antibody IHC of mouse spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)
Hamster anti-Mouse CD69 Monoclonal Antibody | anti-CD69 antibody
Hamster Monoclonal [clone H1.2F3] (IgG,l) to Mouse CD69
Gene Names
CD69; AIM; EA1; MLR-3; CLEC2C; GP32/28; BL-AC/P26
Reactivity
Mouse
Applications
Immunohistochemistry, Immunoprecipitation, Flow Cytometry, Functional Assay
Purity
Purified
Synonyms
CD69; Monoclonal Antibody; Hamster Monoclonal [clone H1.2F3] (IgG; l) to Mouse CD69; Anti-CD69 Antibody (clone H1.2F3; FITC) IHC-plus; Activation inducer molecule; AIM; CD69 antigen; CLEC2C; EA1; Early activation antigen CD69; BL-AC/P26; CD69 molecule; gp32/28; MLR-3; Mouse CD69; anti-CD69 antibody
Host
Hamster
Reactivity
Mouse
Clonality
Monoclonal
Isotype
IgG,l
Clone Number
H1.2F3
Specificity
Recognizes mouse CD69/Very Early Activation Antigen (VEA), Mr 85kD (unreduced).
Purity/Purification
Purified
Form/Format
PBS, 0.1% sodium azide.
Concentration
0.5 mg/ml (varies by lot)
Sequence Length
199
Applicable Applications for anti-CD69 antibody
Immunohistochemistry (IHC - Paraffin), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
Application Notes
Flo, IHC-P (10 ug/ml), IP
Usage: Flow cytometry. Immunoprecipitation. In vitro T cell and NK-cell activation. Flow Cytometry: 1 ug/1E6 cells.
Usage: Flow cytometry. Immunoprecipitation. In vitro T cell and NK-cell activation. Flow Cytometry: 1 ug/1E6 cells.
Target Species
Mouse
Immunogen Description
Y245 mouse dendritic epidermal T cell line.
Immunogen
CD69 antibody was raised against y245 mouse dendritic epidermal T cell line.
Antibody Modification
FITC
Preparation and Storage
+4 degree C (short-term), -20 degree C (long-term). Protect from light.
Immunohistochemistry (IHC)
(Anti-CD69 antibody IHC of mouse spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)
Immunohistochemistry (IHC)
(Anti-CD69 antibody IHC of mouse spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
22,559 Da
NCBI Official Full Name
early activation antigen CD69
NCBI Official Synonym Full Names
CD69 molecule
NCBI Official Symbol
CD69
NCBI Official Synonym Symbols
AIM; EA1; MLR-3; CLEC2C; GP32/28; BL-AC/P26
NCBI Protein Information
early activation antigen CD69; leukocyte surface antigen Leu-23; early T-cell activation antigen p60; early lymphocyte activation antigen; activation inducer molecule (AIM/CD69); C-type lectin domain family 2, member C; CD69 antigen (p60, early T-cell activation antigen)
UniProt Protein Name
Early activation antigen CD69
Protein Family
UniProt Gene Name
CD69
UniProt Synonym Gene Names
CLEC2C; AIM
UniProt Entry Name
CD69_HUMAN
NCBI Description
This gene encodes a member of the calcium dependent lectin superfamily of type II transmembrane receptors. Expression of the encoded protein is induced upon activation of T lymphocytes, and may play a role in proliferation. Furthermore, the protein may act to transmit signals in natural killer cells and platelets. [provided by RefSeq, Aug 2011]
Uniprot Description
Involved in lymphocyte proliferation and functions as a signal transmitting receptor in lymphocytes, natural killer (NK) cells, and platelets.
Research Articles on CD69
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Product Notes
The CD69 cd69 (Catalog #AAA245914) is an Antibody produced from Hamster and is intended for research purposes only. The product is available for immediate purchase. The Hamster Monoclonal [clone H1.2F3] (IgG,l) to Mouse CD69 reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD69 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin), Immunoprecipitation (IP), Flow Cytometry (FC/FACS). Flo, IHC-P (10 ug/ml), IP Usage: Flow cytometry. Immunoprecipitation. In vitro T cell and NK-cell activation. Flow Cytometry: 1 ug/1E6 cells. Researchers should empirically determine the suitability of the CD69 cd69 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD69, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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