Human Heterogeneous Nuclear Ribonucleoprotein U (HNRPU) ELISA Kit | HNRPU elisa kit

Human Heterogeneous Nuclear Ribonucleoprotein U (HNRPU) ELISA Kit

Cat. Num. AAA2705692

• Gene Names: Hnrnpu; SN1; Hnrpu; SAF-A; SP120; hnRNP U
• Reactivity: Human
• Synonyms: Heterogeneous Nuclear Ribonucleoprotein U (HNRPU); Human Heterogeneous Nuclear Ribonucleoprotein U (HNRPU) ELISA Kit; hnRNP-U; HNRNPU; SAF-A; U21.1; Scaffold Attachment Factor A; HNRPU elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Heterogeneous Nuclear Ribonucleoprotein U (HNRPU).; No significant cross-reactivity or interference between Heterogeneous Nuclear Ribonucleoprotein U (HNRPU) and analogues was observed.
• Sequence Length: 798

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.057ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Heterogeneous Nuclear Ribonucleoprotein U (HNRPU) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Heterogeneous Nuclear Ribonucleoprotein U (HNRPU) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HNRPU elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heterogeneous Nuclear Ribonucleoprotein U (HNRPU). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Heterogeneous Nuclear Ribonucleoprotein U (HNRPU). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heterogeneous Nuclear Ribonucleoprotein U (HNRPU), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Heterogeneous Nuclear Ribonucleoprotein U (HNRPU) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 148747541
• NCBI GeneID: 117280
• NCBI Accession #: NP_476480.2
• NCBI GenBank Nucleotide #: NM_057139.2
• UniProt Accession #: Q6IMY8
• Molecular Weight: 87,732 Da
• NCBI Official Full Name: heterogeneous nuclear ribonucleoprotein U
• NCBI Official Synonym Full Names: heterogeneous nuclear ribonucleoprotein U
• NCBI Official Symbol: Hnrnpu
• NCBI Official Synonym Symbols: SN1; Hnrpu; SAF-A; SP120; hnRNP U
• NCBI Protein Information: heterogeneous nuclear ribonucleoprotein U
• UniProt Protein Name: Heterogeneous nuclear ribonucleoprotein U
• UniProt Gene Name: Hnrnpu
• UniProt Synonym Gene Names: SAF-A

NCBI Description

may be involved in glutamate transport and synaptogenesis [RGD, Feb 2006]

Uniprot Description

DNA- and RNA-binding protein involved in several cellular processes such as nuclear chromatin organization, telomere-length regulation, transcription, mRNA alternative splicing and stability, Xist-mediated transcriptional silencing and mitotic cell progression. Plays a role in the regulation of interphase large-scale gene-rich chromatin organization through chromatin-associated RNAs (caRNAs) in a transcription-dependent manner, and thereby maintains genomic stability. Required for the localization of the long non-coding Xist RNA on the inactive chromosome X (Xi) and the subsequent initiation and maintenance of X-linked transcriptional gene silencing during X-inactivation (). Required for the topoisomerase TOP2A protein stability and activity in a RNA-dependent manner (PubMed:20554522). Plays a role as a RNA polymerase II (Pol II) holoenzyme transcription regulator. Promotes transcription initiation by direct association with the core-TFIIH basal transcription factor complex for the assembly of a functional pre-initiation complex with Pol II in a actin-dependent manner. Blocks Pol II transcription elongation activity by inhibiting the C-terminal domain (CTD) phosphorylation of Pol II and dissociates from Pol II pre-initiation complex prior to productive transcription elongation. Positively regulates CBX5-induced transcriptional gene silencing and retention of CBX5 in the nucleus. Negatively regulates glucocorticoid-mediated transcriptional activation. Key regulator of transcription initiation and elongation in embryonic stem cells upon leukemia inhibitory factor (LIF) signaling. Involved in the long non-coding RNA H19-mediated Pol II transcriptional repression. Participates in the circadian regulation of the core clock component ARNTL/BMAL1 transcription. Plays a role in the regulation of telomere length. Plays a role as a global pre-mRNA alternative splicing modulator by regulating U2 small nuclear ribonucleoprotein (snRNP) biogenesis. Plays a role in mRNA stability. Component of the CRD-mediated complex that promotes MYC mRNA stabilization. Enhances the expression of specific genes, such as tumor necrosis factor TNFA, by regulating mRNA stability, possibly through binding to the 3'-untranslated region (UTR). Plays a role in mitotic cell cycle regulation. Involved in the formation of stable mitotic spindle microtubules (MTs) attachment to kinetochore, spindle organization and chromosome congression. Phosphorylation at Ser-58 by PLK1 is required for chromosome alignement and segregation and progression through mitosis. Contributes also to the targeting of AURKA to mitotic spindle MTs (). Binds to double- and single-stranded DNA and RNA, poly(A), poly(C) and poly(G) oligoribonucleotides (PubMed:20554522). Binds to chromatin-associated RNAs (caRNAs) (). Associates with chromatin to scaffold/matrix attachment region (S/MAR) elements in DNA (PubMed:8509422). Associates with chromatin in a chromatin-associated RNAs (caRNAs)-dependent manner. Binds to the Xist RNA. Binds the long non-coding H19 RNA. Binds to SMN1/2 pre-mRNAs at G/U-rich regions. Binds to small nuclear RNAs (snRNAs). Binds to the 3'-UTR of TNFA mRNA. Binds (via RNA-binding RGG-box region) to the long non-coding Xist RNA; this binding is direct and bridges the Xist RNA and the inactive chromosome X (Xi). Also negatively regulates embryonic stem cell differentiation upon LIF signaling. Required for embryonic development ().