Typical Testing Data/Standard Curve (for reference only)
Human Hepcidin 25 (Hepc25) ELISA Kit | Hepc25 elisa kit
Human Hepcidin 25, Hepc25 ELISA Kit
Reactivity
Human
Synonyms
Hepcidin 25 (Hepc25); Human Hepcidin 25; Hepc25 ELISA Kit; Human Hepcidin 25 (Hepc25) ELISA Kit; Hepc25 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of mouse Hepc. No significant cross-reactivity or interference between mouse Hepc and analogues was observed.
Samples
Serum, Plasma, Tissue Homogenates
Assay Type
Quantitative Sandwich
Detection Range
0.78 ng/ml-50 ng/ml
Sensitivity
0.195 ng/ml.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Hepc25 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for Hepc has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Hepc present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for Hepc is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Hepc bound in the initial step. The color development is stopped and the intensity of the color is measured.
Similar Products
Product Notes
The Human Hepc25 (Catalog #AAA700759) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA700759 ELISA Kit recognizes Human Hepc25. It is sometimes possible for the material contained within the vial of "Hepcidin 25 (Hepc25), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
