Rabbit SNAI2 Polyclonal Antibody | anti-SNAI2 antibody

SNAI2 Polyclonal Antibody

Cat. Num. AAA3006771

• Gene Names: SNAI2; SLUG; WS2D; SLUGH; SLUGH1; SNAIL2
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: SNAI2; Polyclonal Antibody; SNAI2 Polyclonal Antibody; Zinc finger protein SNAI2; Neural crest transcription factor Slug; Protein snail homolog 2; SLUG; SLUGH; anti-SNAI2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: SNAI2 polyclonal antibody detects endogenous levels of SNAI2 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 268

• Applicable Applications for anti-SNAI2 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: A synthetic peptide corresponding to residues in Human SNAI2.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of SNAI2 polyclonal antibody at 1:500 dilutionLane1:HEK293T whole cell lysateLane2:NIH-3T3 whole cell lysateLane3:PC12 whole cell lysate)

Related Product Information for anti-SNAI2 antibody

The SNAIL family of developmental regulatory proteins is a group of widely conserved zinc-finger proteins that regulate transcription and include the mammalian proteins SLUG, SNAI 1 (the human homolog of Drosophila SNAIL) and Smuc. SNAI 1 and SLUG are expressed in placenta and in adult heart, liver, and skeletal muscle. SNAI 1, and the corresponding mouse homolog Sna, each contain three classic zinc fingers and one atypical zinc finger, while SLUG contains five zinc finger regions and a transcriptional repression domain at the amino terminus, which enables SLUG to act as a negative regulator of gene expression. SLUG is implicated in the generation and migration of neural crest cells in human embryos and also contributes to limb bud development. In addition, SLUG also constitutes a cellular anti-apoptotic transcription factor that effectively prevents apoptosis in murine pro-B cells deprived of IL-3. The SNAIL-related gene from murine skeletal muscle cells, Smuc, is highly expressed in skeletal muscle and thymus and can, likewise, repress gene transcription. Smuc preferentially associates with CAGGTG and CACCTG E-box motifs (CANNTG) on DNA and involves the five putative DNA-binding zinc finger domains at the C-terminal region of Smuc.

NCBI and Uniprot Product Information

• NCBI GI #: 11134406
• NCBI GeneID: 6591
• UniProt Accession #: O43623
• Molecular Weight: ~ 30kDa
• NCBI Official Full Name: Zinc finger protein SNAI2
• NCBI Official Synonym Full Names: snail family transcriptional repressor 2
• NCBI Official Symbol: SNAI2
• NCBI Official Synonym Symbols: SLUG; WS2D; SLUGH; SLUGH1; SNAIL2
• NCBI Protein Information: zinc finger protein SNAI2
• UniProt Protein Name: Zinc finger protein SNAI2
• Protein Family: Zinc finger protein
• UniProt Gene Name: SNAI2
• UniProt Synonym Gene Names: SLUG; SLUGH
• UniProt Entry Name: SNAI2_HUMAN

NCBI Description

This gene encodes a member of the Snail family of C2H2-type zinc finger transcription factors. The encoded protein acts as a transcriptional repressor that binds to E-box motifs and is also likely to repress E-cadherin transcription in breast carcinoma. This protein is involved in epithelial-mesenchymal transitions and has antiapoptotic activity. Mutations in this gene may be associated with sporatic cases of neural tube defects. [provided by RefSeq, Jul 2008]

Uniprot Description

Snail2: Transcriptional repressor. Involved in the generation and migration of neural crest cells. Plays a role in mediating RAF1-induced transcriptional repression of the TJ protein, occludin (OCLN) and subsequent oncogenic transformation of epithelial cells. Interacts (via SNAG domain) with LIMD1 (via LIM domains), WTIP (via LIM domains) and AJUBA (via LIM domains). Expressed in placenta and adult heart, pancreas, liver, kidney and skeletal muscle. Belongs to the snail C2H2-type zinc-finger protein family.

Protein type: C2H2-type zinc finger protein; Transcription factor; Apoptosis; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 8q11

Cellular Component: cytoplasm; nuclear chromatin; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; sequence-specific DNA binding; metal ion binding; chromatin binding

Biological Process: transcription from RNA polymerase II promoter; substrate-bound cell migration, cell release from substrate; Notch signaling pathway; neural crest cell development; regulation of osteoblast differentiation; negative regulation of chondrocyte differentiation; palate development; negative regulation of transcription from RNA polymerase II promoter; Wnt receptor signaling pathway through beta-catenin; osteoblast differentiation; negative regulation of DNA damage response, signal transduction by p53 class mediator; sensory perception of sound; pigmentation; white fat cell differentiation; positive regulation of fat cell differentiation; epithelial to mesenchymal transition; positive regulation of histone acetylation; negative regulation of cell adhesion mediated by integrin; regulation of chemokine production; positive regulation of cell migration

Disease: Waardenburg Syndrome, Type 2d; Piebald Trait