Human Heat Shock Protein 40 (HSP40) ELISA Kit | HSP40 elisa kit

Human Heat Shock Protein 40 (HSP40) ELISA Kit

Cat. Num. AAA454869

• Gene Names: DNAJB1; Hdj1; Sis1; HSPF1; Hsp40; RSPH16B
• Reactivity: Human
• Synonyms: Heat Shock Protein 40 (HSP40); Human Heat Shock Protein 40 (HSP40) ELISA Kit; DnaJ; DNAJB1; Sis1; RSPH16B; HSPF1; DnaJ homolog subfamily B member 1; Radial Spoke 16 Homolog B; Heat Shock 40kDa Protein 1; DnaJ protein homolog 1; HSP40 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of HSP40. No significant cross-reactivity or interference between HSP40 and analogues was observed.
• Sequence Length: 240

• Samples: Human tissue homogenates, cell lysates or other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: <0.043ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level HSP40 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level HSP40 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HSP40 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of HSP40 in human tissue homogenates, cell lysates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to HSP40. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to HSP40. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain HSP40, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of HSP40 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 665505950
• NCBI GeneID: 3337
• NCBI Accession #: NP_001287843.1
• NCBI GenBank Nucleotide #: NM_001300914.1
• UniProt Accession #: P25685; B4DX52
• Molecular Weight: 27,016 Da
• NCBI Official Full Name: dnaJ homolog subfamily B member 1 isoform 2
• NCBI Official Synonym Full Names: DnaJ heat shock protein family (Hsp40) member B1
• NCBI Official Symbol: DNAJB1
• NCBI Official Synonym Symbols: Hdj1; Sis1; HSPF1; Hsp40; RSPH16B
• NCBI Protein Information: dnaJ homolog subfamily B member 1
• UniProt Protein Name: DnaJ homolog subfamily B member 1
• UniProt Gene Name: DNAJB1
• UniProt Synonym Gene Names: DNAJ1; HDJ1; HSPF1; HSP40; Heat shock protein 40; hDj-1

NCBI Description

This gene encodes a member of the DnaJ or Hsp40 (heat shock protein 40 kD) family of proteins. DNAJ family members are characterized by a highly conserved amino acid stretch called the 'J-domain' and function as one of the two major classes of molecular chaperones involved in a wide range of cellular events, such as protein folding and oligomeric protein complex assembly. The encoded protein is a molecular chaperone that stimulates the ATPase activity of Hsp70 heat-shock proteins in order to promote protein folding and prevent misfolded protein aggregation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]

Uniprot Description

Interacts with HSP70 and can stimulate its ATPase activity. Stimulates the association between HSC70 and HIP.