Human Heat Shock 70kDa Protein 12B (HSPA12B) ELISA Kit | HSPA12B elisa kit

Human Heat Shock 70kDa Protein 12B (HSPA12B) ELISA Kit

Cat. Num. AAA455534

• Gene Names: HSPA12B; C20orf60
• Reactivity: Human
• Synonyms: Heat Shock 70kDa Protein 12B (HSPA12B); Human Heat Shock 70kDa Protein 12B (HSPA12B) ELISA Kit; C20orf60; HSPA12B elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of HSPA12B. No significant cross-reactivity or interference between HSPA12B and analogues was observed.
• Sequence Length: 685

• Samples: Tissue homogenates, cell lysates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.312-20ng/mL
• Sensitivity: 0.128ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level HSPA12B were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level HSPA12B were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HSPA12B elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of HSPA12B in human tissue homogenates, cell lysates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to HSPA12B. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to HSPA12B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain HSPA12B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of HSPA12B in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 309747075
• NCBI GeneID: 116835
• NCBI Accession #: NP_001184256.1
• NCBI GenBank Nucleotide #: NM_001197327.1
• UniProt Accession #: Q96MM6; Q2TAK3; Q9BR52; D3DVX7
• Molecular Weight: 75,688 Da
• NCBI Official Full Name: heat shock 70 kDa protein 12B isoform 2
• NCBI Official Synonym Full Names: heat shock protein family A (Hsp70) member 12B
• NCBI Official Symbol: HSPA12B
• NCBI Official Synonym Symbols: C20orf60
• NCBI Protein Information: heat shock 70 kDa protein 12B
• UniProt Protein Name: Heat shock 70 kDa protein 12B
• Protein Family: Heat shock 70 kDa protein
• UniProt Gene Name: HSPA12B
• UniProt Synonym Gene Names: C20orf60

NCBI Description

The protein encoded by this gene contains an atypical heat shock protein 70 (Hsp70) ATPase domain and is therefore a distant member of the mammalian Hsp70 family. This gene may be involved in susceptibility to atherosclerosis. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2015]