ASAM Active Protein | CLMP active protein

ASAM Protein, Rat, Recombinant (His Tag)

Cat. Num. AAA8123382

• Gene Names: Clmp; ACAM; Asam; Ol16
• Purity: >85% as determined by SDS-PAGE
• Synonyms: ASAM; ASAM Protein; Rat; Recombinant (His Tag); Rat ASAM/CLMP Protein (His Tag); ACAM Protein; Asam Protein; Ol16 Protein; CXADR-like membrane protein; CLMP active protein

• Host: HEK293 Cells
• Purity/Purification: >85% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Met232

• Species: Rat
• Activity: Measured by the ability of the immobilized protein to support the adhesion of MS-1 cells. When 5 x 10E4 cells/well are added to Recombinant Rat ASAM coated plates (12.5ug/mL with 100uL/well), 35-70% cells will adhere after 1 hour incubation at 37 degree C.
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Thr 18
• Tag: C-His
• Protein Construction: A DNA sequence encoding the rat ASAM (Q8K1G0)(Met1-Met232) was expressed, fused with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for CLMP active protein

Background: Adipocyte-specific adhesion molecule (ASAM), also known as ACAM and CLMP, is a type I transmembrane protein and a member of the CTX (cortical thymocyte marker in Xenopus) family within the immunoglobulin superfamily. ASAM protein is highly expressed in the small intestine and placenta, and is found at intermediate levels in the heart, skeletal muscle, colon, spleen, kidney, and lung, and appears in low levels in the liver and peripheral blood leukocytes as well. ASAM is a transmembrane component of tight junctions in epithelial cells that can mediate cell aggregation and regulate transepithelial resistance across polarized epithelial cells. In addition, its expression is strongly correlated with white adipose tissue (WAT) mass of human and rodents with obesity.

References

Eguchi J, et al. (2005) Identification of adipocyte adhesion molecule (ACAM), a novel CTX gene family, implicated in adipocyte maturation and development of obesity. Biochem J. 387(Pt 2): 343-53.Sze KL, et al. (2008) Expression of CLMP, a novel tight junction protein, is mediated via the interaction of GATA with the Kruppel family proteins, KLF4 and Sp1, in mouse TM4 Sertoli cells. J Cell Physiol. 214(2): 334-44.Sze KL, et al. (2008) Post-transcriptional regulation of CLMP mRNA is controlled by tristetraprolin in response to TNFalpha via c-Jun N-terminal kinase signalling. Biochem J. 410(3): 575-83.

NCBI and Uniprot Product Information

• NCBI GI #: 35215304
• NCBI GeneID: 286939
• NCBI Accession #: NP_775177.1
• NCBI GenBank Nucleotide #: NM_173154.1
• UniProt Accession #: Q8K1G0
• Molecular Weight: 41,148 Da
• NCBI Official Full Name: CXADR-like membrane protein
• NCBI Official Synonym Full Names: CXADR-like membrane protein
• NCBI Official Symbol: Clmp
• NCBI Official Synonym Symbols: ACAM; Asam; Ol16
• NCBI Protein Information: CXADR-like membrane protein
• UniProt Protein Name: CXADR-like membrane protein
• Protein Family: CXADR-like membrane protein
• UniProt Gene Name: Clmp
• UniProt Synonym Gene Names: Acam; Asam; CAR-like membrane protein

NCBI Description

human homolog plays a role in cell-cell adhesion [RGD, Feb 2006]

Uniprot Description

May be involved in the cell-cell adhesion. May play a role in adipocyte differentiation and development of obesity. Is required for normal small intestine development ().