Typical Testing Data/Standard Curve (for reference only)
Human H-Cadherin ELISA Kit | H-Cadherin elisa kit
Human H-Cadherin (Cadherin, Heart) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human H-Cadherin. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human H-Cadherin and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human H-Cadherin, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human H-Cadherin. You can calculate the concentration of Human H-Cadherin in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
Uniprot Description
CDH13: Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. May act as a negative regulator of neural cell growth. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Cell adhesion; Membrane protein, GPI anchor
Chromosomal Location of Human Ortholog: 16q23.3
Cellular Component: extracellular space; neuron projection; focal adhesion; cytoplasm; plasma membrane; caveola; external side of plasma membrane
Molecular Function: adiponectin binding; protein homodimerization activity; cadherin binding; low-density lipoprotein binding; calcium ion binding
Biological Process: lamellipodium biogenesis; intercellular junction assembly and maintenance; positive regulation of positive chemotaxis; positive regulation of smooth muscle cell proliferation; Rac protein signal transduction; positive regulation of calcium-mediated signaling; negative regulation of cell adhesion; keratinocyte proliferation; regulation of epidermal growth factor receptor signaling pathway; Rho protein signal transduction; low density lipoprotein mediated signaling; localization within membrane; negative regulation of cell proliferation; calcium-dependent cell-cell adhesion; regulation of endocytosis; endothelial cell migration; positive regulation of endothelial cell proliferation; positive regulation of transcription from RNA polymerase II promoter; sprouting angiogenesis; homophilic cell adhesion; positive regulation of cell-matrix adhesion; positive regulation of cell migration
Similar Products
Product Notes
The Human H-Cadherin cdh13 (Catalog #AAA2500112) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2500112 ELISA Kit recognizes Human H-Cadherin. It is sometimes possible for the material contained within the vial of "H-Cadherin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
