Human Guanylate Cyclase Activator 2B (GUCA2B) ELISA Kit | GUCA2B elisa kit

Human Guanylate Cyclase Activator 2B (GUCA2B) ELISA Kit

Cat. Num. AAA2706059

• Reactivity: Human
• Synonyms: Guanylate Cyclase Activator 2B (GUCA2B); Human Guanylate Cyclase Activator 2B (GUCA2B) ELISA Kit; UGN; GCAP-II; Uroguanylin; Guanylate cyclase C-activating peptide II; GUCA2B elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Guanylate Cyclase Activator 2B (GUCA2B).; No significant cross-reactivity or interference between Guanylate Cyclase Activator 2B (GUCA2B) and analogues was observed.
• Sequence Length: 113

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue Homogenates and Other Biological Fluids
• Detection Range: 15.6-1,000pg/mL
• Sensitivity: < 5.6pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Guanylate Cyclase Activator 2B (GUCA2B) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Guanylate Cyclase Activator 2B (GUCA2B) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GUCA2B elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Guanylate Cyclase Activator 2B (GUCA2B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Guanylate Cyclase Activator 2B (GUCA2B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Guanylate Cyclase Activator 2B (GUCA2B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Guanylate Cyclase Activator 2B (GUCA2B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 521025864
• UniProt Accession #: O13009
• Molecular Weight: 12,044 Da
• NCBI Official Full Name: Guanylate cyclase activator 2B
• UniProt Protein Name: Guanylate cyclase activator 2B
• Protein Family: Guanylate cyclase activator
• UniProt Gene Name: GUCA2B
• UniProt Synonym Gene Names: UGN

Uniprot Description

Endogenous activator of intestinal guanylate cyclase. It stimulates this enzyme through the same receptor binding region as the heat-stable enterotoxins. May be a potent physiological regulator of intestinal fluid and electrolyte transport. May be an autocrine/paracrine regulator of intestinal salt and water transport ().