Typical Testing Data/Standard Curve (for reference only)
Human GPR116 (G-protein coupled receptor 116) ELISA Kit | GPR116 elisa kit
Human GPR116 (G-protein coupled receptor 116) ELISA Kit
Gene Names
ADGRF5; GPR116; KPG_001
Reactivity
Human
Synonyms
GPR116 (G-protein coupled receptor 116); Human GPR116 (G-protein coupled receptor 116) ELISA Kit; GPR116 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection ofGPR116. No significant cross-reactivity or interference betweenGPR116and analogues was observed.
Sequence Length
1346
Samples
serum, plasma, tissue homogenatesand other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
0.313-20ng/ml
Sensitivity
<0.188ng/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high levelGPR116were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high levelGPR116were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<10%
Preparation and Storage
Store at 4 degree C for 6 months.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for GPR116 elisa kit
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- GPR116antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-GPR116antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the GPR116amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofGPR116can be calculated.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
133,307 Da
NCBI Official Full Name
G protein coupled receptor 116
NCBI Official Synonym Full Names
adhesion G protein-coupled receptor F5
NCBI Official Symbol
ADGRF5
NCBI Official Synonym Symbols
GPR116; KPG_001
NCBI Protein Information
adhesion G protein-coupled receptor F5
UniProt Protein Name
Adhesion G protein-coupled receptor F5
UniProt Gene Name
ADGRF5
Uniprot Description
Receptor that plays a critical role in lung surfactant homeostasis. May play a role in controlling adipocyte function.
Research Articles on GPR116
Similar Products
Product Notes
The Human GPR116 adgrf5 (Catalog #AAA7607073) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7607073 ELISA Kit recognizes Human GPR116. It is sometimes possible for the material contained within the vial of "GPR116 (G-protein coupled receptor 116), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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