Human E3 Ubiquitin-Protein Ligase UBR4 (UBR4) ELISA Kit | UBR4 elisa kit

Human E3 Ubiquitin-Protein Ligase UBR4 (UBR4) ELISA Kit

Cat. Num. AAA9713762

• Gene Names: UBR4; p600; ZUBR1; RBAF600
• Reactivity: Human
• Synonyms: E3 Ubiquitin-Protein Ligase UBR4 (UBR4); Human E3 Ubiquitin-Protein Ligase UBR4 (UBR4) ELISA Kit; UBR4; RP5-1126H10.1; FLJ41863; KIAA0462; KIAA1307; RBAF600; ZUBR1; p600; retinoblastoma-associated factor 600; retinoblastoma-associated factor 600-like protein; zinc finger; UBR1 type 1; UBR4 elisa kit

• Reactivity: Human
• Specificity: Human E3 ubiquitin-protein ligase UBR4 (UBR4) ELISA Kit has high sensitivity and excellent specificity for detection of Human UBR4. No significant cross-reactivity or interference between Human UBR4 and analogues was observed.
• Sequence Length: 5183

• Assay Type: Sandwich ELISA (Quantitative)
• Samples: Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
• Detection Method: Colorimetric
• Usage Notes: * Do not mix components from different kit lots or use reagents beyond the kit expiration date.; * Allow all reagents to warm to room temperature for at least 30 minutes before opening.; * Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.; * Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.; * Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.; * It is recommended that all samples and standards be assayed in duplicate or triplicate.
• Preparation and Storage: Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Human E3 ubiquitin-protein ligase UBR4 (UBR4) Standard Curve.)

Related Product Information for UBR4 elisa kit

Description: This Human E3 ubiquitin-protein ligase UBR4 (UBR4) ELISA Kit employs a two-site sandwich ELISA to quantitate UBR4 in samples. An antibody specific for UBR4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any UBR4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for UBR4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of UBR4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: RBAF600 associates with RB in the nucleus and with Ca(2+)-bound calmodulin in the cytoplasm.p600 and RB appears to act as a chromatin scaffold, and in the cytoplasm p600 and clathrin form a meshwork structure which could contribute to cytoskeletal organization and membrane morphogenesis. Reduced expression of p600 with interfering RNA abrogated integrin-mediated ruffled membrane formation and prevented activation of integrin-mediated survival pathways. The deduced 5,183-amino acid protein has a calculated molecular mass of 573.5 kD. Immunostaining of human foreskin fibroblasts localized p600 in a continuous meshwork pattern from the nucleus to the cytoplasm, passing through the nuclear envelope. In the cytoplasm, p600 was concentrated at the leading edge of membrane structures.

NCBI and Uniprot Product Information

• NCBI GI #: 82659109
• NCBI GeneID: 23352
• NCBI Accession #: NP_065816.2
• NCBI GenBank Nucleotide #: NP_065816.2
• UniProt Accession #: Q5T4S7
• NCBI Official Full Name: E3 ubiquitin-protein ligase UBR4
• NCBI Official Synonym Full Names: ubiquitin protein ligase E3 component n-recognin 4
• NCBI Official Symbol: UBR4
• NCBI Official Synonym Symbols: p600; ZUBR1; RBAF600
• NCBI Protein Information: E3 ubiquitin-protein ligase UBR4
• UniProt Protein Name: E3 ubiquitin-protein ligase UBR4
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: UBR4
• UniProt Synonym Gene Names: KIAA0462; KIAA1307; RBAF600; ZUBR1; RBAF600; p600

NCBI Description

The protein encoded by this gene is an E3 ubiquitin-protein ligase that interacts with the retinoblastoma-associated protein in the nucleus and with calcium-bound calmodulin in the cytoplasm. The encoded protein appears to be a cytoskeletal component in the cytoplasm and part of the chromatin scaffold in the nucleus. In addition, this protein is a target of the human papillomavirus type 16 E7 oncoprotein. [provided by RefSeq, Aug 2010]

Uniprot Description

E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation. Together with clathrin, forms meshwork structures involved in membrane morphogenesis and cytoskeletal organization. Regulates integrin-mediated signaling. May play a role in activation of FAK in response to cell-matrix interactions. Mediates ubiquitination of ACLY, leading to its subsequent degradation.