Human GM-CSF ELISA Kit | GM-CSF elisa kit

Human GM-CSF ELISA Kit

Cat. Num. AAA335689

• Gene Names: CSF2; GMCSF
• Reactivity: Human
• Synonyms: GM-CSF; Human GM-CSF ELISA Kit; GM-CSF elisa kit

• Reactivity: Human
• Specificity: The assay recognizes natural human GM-CSF. To define specificity of this ELISA, several proteins were tested for cross reactivity. There was no cross reactivity observed for any protein tested (IL-1alpha, IL-1beta, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IFNgamma, TNFalpha).
• Sequence Length: 144

• Samples: Cell Culture Supernatants, Human serum, plasma
• Sensitivity: The sensitivity, minimum detectable dose of GM-CSF using this GM-CSF ELISA kit was found to be <4.8pg/ml. This was determined by adding 2 standard deviations to the mean OD obtained when the zero standard was assayed 40 times.
• Intra-Assay: Reproducibility within the assay was evaluated in three independent experiments. Each assay was carried out with 6 replicates (3 duplicates) in 2 human pooled serum, 2 in culture media and 2 in standard diluent with samples containing different concentrations of GM-CSF. 2 standard curves were run on each plate The overall intra-assay coefficient of variation has been calculated to be 3.7%.
• Inter-Assay: Assay to assay reproducibility within one laboratory was evaluated in three independent experiments by two technicians. Each assay was carried out with 6 replicates (3 duplicates) in 2 human pooled serum, 2 in culture media and 2 in standard diluent with samples containing different concentrations of GM-CSF. 2 standard curves were run on each plate. The calculated overall coefficient of variation was 6.6%.
• Intended Uses: The GM-CSF ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of soluble GM-CSF levels in cell culture supernatants, human serum, plasma.

Related Product Information for GM-CSF elisa kit

Principle of the assay: A capture Antibody highly specific for GM-CSF has been coated to the wells of the microtiter strip plate provided during manufacture. Binding of GM-CSF in samples and known standards to the capture antibodies and subsequent binding of the biotinylated anti- GMCSF secondary antibody to the analyte is completed during the same incubation period. Any excess unbound analyte and secondary antibody is removed. The HRP conjugate solution is then added to every well including the zero wells, following incubation excess conjugate is removed by careful washing. A chromogen substrate is added to the wells resulting in the progressive development of a blue coloured complex with the conjugate. The colour development is then stopped by the addition of acid turning the resultant final product yellow. The intensity of the produced coloured complex is directly proportional to the concentration of GM-CSF present in the samples and standards. The absorbance of the colour complex is then measured and the generated OD values for each standard are plotted against expected concentration forming a standard curve. This standard curve can then be used to accurately determine the concentration of GM-CSF in any sample tested.

NCBI and Uniprot Product Information

• NCBI GI #: 183364
• NCBI GeneID: 1437
• Molecular Weight: 16,295 Da
• NCBI Official Full Name: GM-CSF
• NCBI Official Synonym Full Names: colony stimulating factor 2 (granulocyte-macrophage)
• NCBI Official Symbol: CSF2
• NCBI Official Synonym Symbols: GMCSF
• NCBI Protein Information: granulocyte-macrophage colony-stimulating factor
• UniProt Protein Name: Granulocyte-macrophage colony-stimulating factor
• UniProt Gene Name: CSF2
• UniProt Synonym Gene Names: GMCSF; GM-CSF; CSF
• UniProt Entry Name: CSF2_HUMAN

NCBI Description

The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13. [provided by RefSeq, Jul 2008]

Uniprot Description

GMCSF: Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. Belongs to the GM-CSF family.

Protein type: Secreted, signal peptide; Cytokine; Secreted

Chromosomal Location of Human Ortholog: 5q31.1

Cellular Component: extracellular space; extracellular region

Molecular Function: protein binding; growth factor activity; granulocyte macrophage colony-stimulating factor receptor binding; cytokine activity

Biological Process: negative regulation of cytolysis; epithelial fluid transport; macrophage activation; embryonic placenta development; positive regulation of tyrosine phosphorylation of Stat5 protein; positive regulation of interleukin-23 production; positive regulation of cell proliferation; myeloid dendritic cell differentiation; immune response; positive regulation of DNA replication