Human Glycosylphosphatidylinositol Anchored High Density Lipoprotein Binding Protein 1 (GPIHBP1) ELISA Kit | GPIHBP1 elisa kit

Human Glycosylphosphatidylinositol Anchored High Density Lipoprotein Binding Protein 1 (GPIHBP1) ELISA Kit

Cat. Num. AAA2021457

• Gene Names: GPIHBP1; HYPL1D; GPI-HBP1
• Reactivity: Human
• Synonyms: Glycosylphosphatidylinositol Anchored High Density Lipoprotein Binding Protein 1 (GPIHBP1); Human Glycosylphosphatidylinositol Anchored High Density Lipoprotein Binding Protein 1 (GPIHBP1) ELISA Kit; GPI-HBP1; High density lipoprotein-binding protein 1; GPIHBP1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Glycosylphosphatidylinositol Anchored High Density Lipoprotein Binding Protein 1 (GPIHBP1).; No significant cross-reactivity or interference between Glycosylphosphatidylinositol Anchored High Density Lipoprotein Binding Protein 1 (GPIHBP1) and analogues was observed.
• Sequence Length: 184

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.625-10ng/mL
• Sensitivity: < 0.242ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glycosylphosphatidylinositol Anchored High Density Lipoprotein Binding Protein 1 (GPIHBP1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glycosylphosphatidylinositol Anchored High Density Lipoprotein Binding Protein 1 (GPIHBP1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GPIHBP1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glycosylphosphatidylinositol Anchored High Density Lipoprotein Binding Protein 1 (GPIHBP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glycosylphosphatidylinositol Anchored High Density Lipoprotein Binding Protein 1 (GPIHBP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glycosylphosphatidylinositol Anchored High Density Lipoprotein Binding Protein 1 (GPIHBP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glycosylphosphatidylinositol Anchored High Density Lipoprotein Binding Protein 1 (GPIHBP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for GPIHBP1 elisa kit

Metabolic pathway

NCBI and Uniprot Product Information

• NCBI GI #: 30794264
• NCBI GeneID: 338328
• NCBI Accession #: NP_835466.1
• NCBI GenBank Nucleotide #: NM_178172.4
• UniProt Accession #: Q8IV16; Q6P3T2; Q86W15
• Molecular Weight: 19,806 Da
• NCBI Official Full Name: glycosylphosphatidylinositol-anchored high density lipoprotein-binding protein 1
• NCBI Official Synonym Full Names: glycosylphosphatidylinositol anchored high density lipoprotein binding protein 1
• NCBI Official Symbol: GPIHBP1
• NCBI Official Synonym Symbols: HYPL1D; GPI-HBP1
• NCBI Protein Information: glycosylphosphatidylinositol-anchored high density lipoprotein-binding protein 1
• UniProt Protein Name: Glycosylphosphatidylinositol-anchored high density lipoprotein-binding protein 1
• Protein Family: Glycosylphosphatidylinositol-anchored high density lipoprotein-binding protein
• UniProt Gene Name: GPIHBP1
• UniProt Synonym Gene Names: HBP1; GPI-HBP1; GPI-anchored HDL-binding protein 1
• UniProt Entry Name: HDBP1_HUMAN

NCBI Description

This gene encodes a capillary endothelial cell protein that facilitates the lipolytic processing of triglyceride-rich lipoproteins. The encoded protein is a glycosylphosphatidylinositol-anchored protein that is a member of the lymphocyte antigen 6 (Ly6) family. This protein plays a major role in transporting lipoprotein lipase (LPL) from the subendothelial spaces to the capillary lumen. Mutations in this gene are the cause of hyperlipoproteinemia, type 1D. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2014]

Uniprot Description

GPIHBP1: Plays a key role in the lipolytic processing of chylomicrons.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 8q24.3

Cellular Component: anchored to external side of plasma membrane; basolateral plasma membrane; apical plasma membrane; external side of plasma membrane

Molecular Function: protein transmembrane transporter activity; high-density lipoprotein binding; lipid binding

Biological Process: intracellular protein transport; cholesterol homeostasis; protein stabilization; protein import; positive regulation of lipoprotein lipase activity; transcytosis; lipid transport

Disease: Hyperlipoproteinemia, Type Id