Human Glutathione S Transferase Mu 3, Brain (GSTm3) ELISA Kit | GSTm3 elisa kit

Human Glutathione S Transferase Mu 3, Brain (GSTm3) ELISA Kit

Cat. Num. AAA2702224

• Gene Names: GSTM3; GST5; GSTB; GTM3; GSTM3-3
• Reactivity: Human
• Synonyms: Glutathione S Transferase Mu 3; Brain (GSTm3); Human Glutathione S Transferase Mu 3; Brain (GSTm3) ELISA Kit; GST-M3; GST5; GSTB; GSTM3-3; GTM3; GSTm3 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Glutathione S Transferase Mu 3, Brain (GSTm3).; No significant cross-reactivity or interference between Glutathione S Transferase Mu 3, Brain (GSTm3) and analogues was observed.
• Sequence Length: 225

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue Homogenates and Other Biological Fluids
• Detection Range: 3.12-200ng/mL
• Sensitivity: < 1.15ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glutathione S Transferase Mu 3, Brain (GSTm3) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glutathione S Transferase Mu 3, Brain (GSTm3) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GSTm3 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glutathione S Transferase Mu 3, Brain (GSTm3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glutathione S Transferase Mu 3, Brain (GSTm3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glutathione S Transferase Mu 3, Brain (GSTm3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glutathione S Transferase Mu 3, Brain (GSTm3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 23065552
• NCBI GeneID: 2947
• NCBI Accession #: NP_000840.2
• NCBI GenBank Nucleotide #: NM_000849.4
• UniProt Accession #: P21266; O60550; Q96HA3
• Molecular Weight: 26,560 Da
• NCBI Official Full Name: glutathione S-transferase Mu 3
• NCBI Official Synonym Full Names: glutathione S-transferase mu 3
• NCBI Official Symbol: GSTM3
• NCBI Official Synonym Symbols: GST5; GSTB; GTM3; GSTM3-3
• NCBI Protein Information: glutathione S-transferase Mu 3
• UniProt Protein Name: Glutathione S-transferase Mu 3
• Protein Family: Glutathione S-transferase
• UniProt Gene Name: GSTM3
• UniProt Synonym Gene Names: GST5; hGSTM3-3

NCBI Description

Cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. This gene encodes a glutathione S-transferase that belongs to the mu class. The mu class of enzymes functions in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. The genes encoding the mu class of enzymes are organized in a gene cluster on chromosome 1p13.3 and are known to be highly polymorphic. These genetic variations can change an individual's susceptibility to carcinogens and toxins as well as affect the toxicity and efficacy of certain drugs. Mutations of this class mu gene have been linked with a slight increase in a number of cancers, likely due to exposure with environmental toxins. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2008]

Uniprot Description

GSTM3: Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. May govern uptake and detoxification of both endogenous compounds and xenobiotics at the testis and brain blood barriers. Belongs to the GST superfamily. Mu family.

Protein type: Cell development/differentiation; EC 2.5.1.18; Other Amino Acids Metabolism - glutathione; Transferase; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Xenobiotic Metabolism - metabolism by cytochrome P450

Chromosomal Location of Human Ortholog: 1p13.3

Cellular Component: cytoplasm; cytosol; nucleus

Molecular Function: enzyme binding; glutathione binding; glutathione transferase activity; identical protein binding; protein homodimerization activity

Biological Process: establishment of blood-nerve barrier; glutathione metabolic process; nitrobenzene metabolic process; response to estrogen; xenobiotic catabolic process