Rabbit Cholesteryl Ester Transfer Protein (CETP) ELISA Kit | CETP elisa kit

Rabbit Cholesteryl Ester Transfer Protein (CETP) ELISA Kit

Cat. Num. AAA454103

• Reactivity: Rabbit
• Synonyms: Cholesteryl Ester Transfer Protein (CETP); Rabbit Cholesteryl Ester Transfer Protein (CETP) ELISA Kit; LTP; Lipid Transfer Protein; Plasma Lipid Transfer Protein; Cholesterylester Transfer Protein; CETP elisa kit

• Reactivity: Rabbit
• Specificity: This assay has high sensitivity and excellent specificity for detection of CETP. No significant cross-reactivity or interference between CETP and analogues was observed.
• Sequence Length: 497

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 93.75-6000pg/mL
• Sensitivity: 42.61pg/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CETP were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CETP were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CETP elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CETP in rabbit serum, plasma and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CETP. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to CETP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CETP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of CETP in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 116121
• NCBI GeneID: 100327267
• UniProt Accession #: P22687
• Molecular Weight: 54,514 Da
• NCBI Official Full Name: Cholesteryl ester transfer protein
• NCBI Official Symbol: CETP
• NCBI Protein Information: cholesteryl ester transfer protein; Lipid transfer protein I; cholesteryl ester transfer protein, plasma
• UniProt Protein Name: Cholesteryl ester transfer protein
• Protein Family: Cholesteryl ester transfer protein
• UniProt Gene Name: CETP

Uniprot Description

Involved in the transfer of neutral lipids, including cholesteryl ester and triglyceride, among lipoprotein particles. Allows the net movement of cholesteryl ester from high density lipoproteins/HDL to triglyceride-rich very low density lipoproteins/VLDL, and the equimolar transport of triglyceride from VLDL to HDL (PubMed:24293641). Regulates the reverse cholesterol transport, by which excess cholesterol is removed from peripheral tissues and returned to the liver for elimination ().