Human Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 2C (GRIN2C) ELISA Kit | GRIN2C elisa kit

Human Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 2C (GRIN2C) ELISA Kit

Cat. Num. AAA452818

• Gene Names: GRIN2C; NR2C; GluN2C; NMDAR2C
• Reactivity: Human
• Synonyms: Glutamate Receptor; Ionotropic; N-Methyl-D-Aspartate 2C (GRIN2C); Human Glutamate Receptor; N-Methyl-D-Aspartate 2C (GRIN2C) ELISA Kit; NMDAR2B; NR2B; hNR3; GRIN2C elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of GRIN2C. No significant cross-reactivity or interference between GRIN2C and analogues was observed.
• Sequence Length: 1,233

• Samples: Tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.312-20ng/mL
• Sensitivity: 0.118ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level GRIN2C were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level GRIN2C were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GRIN2C elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of GRIN2C in human tissue homogenates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to GRIN2C. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to GRIN2C. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain GRIN2C, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of GRIN2C in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 55770854
• NCBI GeneID: 2905
• NCBI Accession #: NP_000826.2
• NCBI GenBank Nucleotide #: NM_000835.4
• UniProt Accession #: Q14957; B2RTT1
• Molecular Weight: 134,209 Da
• NCBI Official Full Name: glutamate receptor ionotropic, NMDA 2C isoform 1
• NCBI Official Synonym Full Names: glutamate ionotropic receptor NMDA type subunit 2C
• NCBI Official Symbol: GRIN2C
• NCBI Official Synonym Symbols: NR2C; GluN2C; NMDAR2C
• NCBI Protein Information: glutamate receptor ionotropic, NMDA 2C
• UniProt Protein Name: Glutamate receptor ionotropic, NMDA 2C
• Protein Family: Glutamate receptor ionotropic
• UniProt Gene Name: GRIN2C
• UniProt Synonym Gene Names: NMDAR2C; GluN2C; NMDAR2C; NR2C

NCBI Description

This gene encodes a subunit of the N-methyl-D-aspartate (NMDA) receptor, which is a subtype of ionotropic glutamate receptor. NMDA receptors are found in the central nervous system, are permeable to cations and have an important role in physiological processes such as learning, memory, and synaptic development. The receptor is a tetramer of different subunits (typically heterodimer of subunit 1 with one or more of subunits 2A-D), forming a channel that is permeable to calcium, potassium, and sodium, and whose properties are determined by subunit composition. Alterations in the subunit composition of the receptor are associated with pathophysiological conditions such as Parkinson's disease, Alzheimer's disease, depression, and schizophrenia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]

Uniprot Description

NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine.