Human Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 2C (GRIN2C) ELISA Kit | GRIN2C elisa kit

Human Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 2C (GRIN2C) ELISA Kit

Cat. Num. AAA2022168

• Gene Names: GRIN2C; NR2C; GluN2C; NMDAR2C
• Reactivity: Human
• Synonyms: Glutamate Receptor; Ionotropic; N-Methyl-D-Aspartate 2C (GRIN2C); Human Glutamate Receptor; N-Methyl-D-Aspartate 2C (GRIN2C) ELISA Kit; NMDAR2C; NR2C; Glutamate [NMDA] receptor subunit epsilon-3; N-methyl D-aspartate receptor subtype 2C; GRIN2C elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 2C (GRIN2C).; No significant cross-reactivity or interference between Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 2C (GRIN2C) and analogues was observed.
• Sequence Length: 1,233

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.118ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 2C (GRIN2C) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 2C (GRIN2C) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GRIN2C elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 2C (GRIN2C). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 2C (GRIN2C). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 2C (GRIN2C), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 2C (GRIN2C) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for GRIN2C elisa kit

Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 55770854
• NCBI GeneID: 2905
• NCBI Accession #: NP_000826.2
• NCBI GenBank Nucleotide #: NM_000835.4
• UniProt Accession #: Q14957; B2RTT1
• Molecular Weight: 134,209 Da
• NCBI Official Full Name: glutamate receptor ionotropic, NMDA 2C isoform 1
• NCBI Official Synonym Full Names: glutamate receptor, ionotropic, N-methyl D-aspartate 2C
• NCBI Official Symbol: GRIN2C
• NCBI Official Synonym Symbols: NR2C; GluN2C; NMDAR2C
• NCBI Protein Information: glutamate receptor ionotropic, NMDA 2C
• UniProt Protein Name: Glutamate receptor ionotropic, NMDA 2C
• Protein Family: Glutamate receptor ionotropic
• UniProt Gene Name: GRIN2C
• UniProt Synonym Gene Names: NMDAR2C; GluN2C; NMDAR2C; NR2C
• UniProt Entry Name: NMDE3_HUMAN

NCBI Description

This gene encodes a subunit of the N-methyl-D-aspartate (NMDA) receptor, which is a subtype of ionotropic glutamate receptor. NMDA receptors are found in the central nervous system, are permeable to cations and have an important role in physiological processes such as learning, memory, and synaptic development. The receptor is a tetramer of different subunits (typically heterodimer of subunit 1 with one or more of subunits 2A-D), forming a channel that is permeable to calcium, potassium, and sodium, and whose properties are determined by subunit composition. Alterations in the subunit composition of the receptor are associated with pathophysiological conditions such as Parkinson's disease, Alzheimer's disease, depression, and schizophrenia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]

Uniprot Description

NMDAR2C: NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2C/GRIN2C subfamily.

Protein type: Channel, calcium; Membrane protein, multi-pass; Membrane protein, integral; Channel, ligand-gated

Chromosomal Location of Human Ortholog: 17q25.1

Cellular Component: postsynaptic membrane; integral to plasma membrane; postsynaptic density; dendrite; plasma membrane; cell junction; N-methyl-D-aspartate selective glutamate receptor complex

Molecular Function: protein binding; extracellular-glutamate-gated ion channel activity; N-methyl-D-aspartate selective glutamate receptor activity; protein N-terminus binding; cation channel activity; PDZ domain binding

Biological Process: synaptic transmission; synaptic transmission, glutamatergic; protein localization; ionotropic glutamate receptor signaling pathway; glutamate signaling pathway; directional locomotion; transport; response to wounding; neuromuscular process controlling balance; regulation of excitatory postsynaptic membrane potential; negative regulation of protein catabolic process