Human Glucocorticoid Induced Tumor Necrosis Factor Receptor (GITR) ELISA Kit | GITR elisa kit

Human Glucocorticoid Induced Tumor Necrosis Factor Receptor (GITR) ELISA Kit

Cat. Num. AAA450755

• Gene Names: TNFRSF18; AITR; GITR; CD357; GITR-D
• Reactivity: Human
• Synonyms: Glucocorticoid Induced Tumor Necrosis Factor Receptor (GITR); Human Glucocorticoid Induced Tumor Necrosis Factor Receptor (GITR) ELISA Kit; TNFRSF18; AITR; Tumor Necrosis Factor Receptor Superfamily 18; Glucocorticoid-induced TNFR-related protein; Activation-inducible TNFR family receptor; GITR elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of GITR. No significant cross-reactivity or interference between GITR and analogues was observed.
• Sequence Length: 241

• Samples: Serum, plasma, tissue homogenates, cell lysates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 15.625-1000pg/mL
• Sensitivity: 5.9pg/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level GITR were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level GITR were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Standard Curve/Testing Data

Related Product Information for GITR elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of GITR in human serum, plasma, tissue homogenates, cell lysates, and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to GITR. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to GITR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain GITR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of GITR in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4759246
• NCBI GeneID: 8784
• NCBI Accession #: NP_004186.1
• NCBI GenBank Nucleotide #: NM_004195.2
• UniProt Accession #: Q9Y5U5; O95851; Q5U0I4; Q9NYJ9; B1AME1
• Molecular Weight: 25,110 Da
• NCBI Official Full Name: tumor necrosis factor receptor superfamily member 18 isoform 1
• NCBI Official Synonym Full Names: TNF receptor superfamily member 18
• NCBI Official Symbol: TNFRSF18
• NCBI Official Synonym Symbols: AITR; GITR; CD357; GITR-D
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 18
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 18
• UniProt Gene Name: TNFRSF18
• UniProt Synonym Gene Names: AITR; GITR

NCBI Description

This gene encodes a member of the TNF-receptor superfamily. The encoded receptor has been shown to have increased expression upon T-cell activation, and it is thought to play a key role in dominant immunological self-tolerance maintained by CD25(+)CD4(+) regulatory T cells. Knockout studies in mice also suggest the role of this receptor is in the regulation of CD3-driven T-cell activation and programmed cell death. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Feb 2011]

Uniprot Description

Receptor for TNFSF18. Seems to be involved in interactions between activated T-lymphocytes and endothelial cells and in the regulation of T-cell receptor-mediated cell death. Mediated NF-kappa-B activation via the TRAF2/NIK pathway.