BVES recombinant protein

BVES Protein, Human, Recombinant (GST Tag)

Cat. Num. AAA8121182

• Gene Names: BVES; POP1; HBVES; LGMD2X; POPDC1
• Purity: >90% as determined by SDS-PAGE
• Synonyms: BVES; BVES Protein; Human; Recombinant (GST Tag); Human BVES/POPDC1 Protein (GST Tag); CARICK Protein; HBVES Protein; LGMD2X Protein; POP1 Protein; POPDC1 Protein; blood vessel epicardial substance; BVES recombinant protein

• Host: E Coli
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Asn36

• Species: Human
• Predicted N Terminal: Met
• Tag: N-GST
• Protein Construction: A DNA sequence encoding the human BVES (NP_671488.1)(Met1-Asn36) was fused with the GST tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for BVES recombinant protein

Background: Blood vessel epicardial substance (BVES), or POPDC1, is a tight junction-associated transmembrane protein that modulates epithelial-to-mesenchymal transition (EMT) via junctional signaling pathways. BVES plays a protective role both in ulcerative and infectious colitis and identify BVES as a critical protector of colonic mucosal integrity. The Popeye domain containing1, also called Bves (Popdc1/Bves), is a transmembrane protein that functions in muscle regeneration, heart rate regulation, hypoxia tolerance, and ischemia preconditioning. The expression of Popdc1/Bves is elevated in cardiomyocytes maintained in serum free defined medium. Popdc1/Bves plays a role in the preservation of cardiomyocyte viability under serum deficiency through the alteration of Rac1 activity and the regulation of Bnip3 expression by FoxO3 and NFkappaB transcription factors pointing to Popdc1/Bves as a potential target to enhance heart protection. Blood vessel epicardial substance (BVES) is a tight junction-associated protein that regulates epithelial-mesenchymal states and is underexpressed in epithelial malignancy. Loss of BVES promotes inflammatory tumourigenesis through dysregulation of Wnt signalling and the oncogene c-Myc. BVES promoter methylation status may serve as a CAC biomarker. Blood vessel epicardial substance (BVES/Popdc1) is a junctional-associated transmembrane protein that is underexpressed in a number of malignancies and regulates epithelial-to-mesenchymal transition. BVES is a key regulator of intestinal stem cell programs and mucosal homeostasis.

NCBI and Uniprot Product Information

• NCBI GI #: 22202606
• NCBI GeneID: 11149
• NCBI Accession #: NP_671488.1
• NCBI GenBank Nucleotide #: NM_147147.3
• UniProt Accession #: Q8NE79; Q5T550; Q5T551; Q8IWC6; Q9HBV0; Q9UNG6; A8K1R4; E1P5D8
• Molecular Weight: 41,451 Da
• NCBI Official Full Name: blood vessel epicardial substance
• NCBI Official Synonym Full Names: blood vessel epicardial substance
• NCBI Official Symbol: BVES
• NCBI Official Synonym Symbols: POP1; HBVES; LGMD2X; POPDC1
• NCBI Protein Information: blood vessel epicardial substance
• UniProt Protein Name: Blood vessel epicardial substance
• Protein Family: Blood vessel epicardial substance
• UniProt Gene Name: BVES
• UniProt Synonym Gene Names: POP1; POPDC1; hBVES; Popeye protein 1
• UniProt Entry Name: POPD1_HUMAN

NCBI Description

This gene encodes a member of the POP family of proteins containing three putative transmembrane domains. This gene is expressed in cardiac and skeletal muscle and may play an important role in development of these tissues. The mouse ortholog may be involved in the regeneration of adult skeletal muscle and may act as a cell adhesion molecule in coronary vasculogenesis. Three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Dec 2010]

Uniprot Description

BVES: Cell adhesion molecule involved in the establishment and/or maintenance of cell integrity. Involved in the formation and regulation of the tight junction (TJ) paracellular permeability barrier in epithelial cells. Plays a role in VAMP3- mediated vesicular transport and recycling of different receptor molecules through its interaction with VAMP3. Plays a role in the regulation of cell shape and movement by modulating the Rho-family GTPase activity through its interaction with ARHGEF25/GEFT. Induces primordial adhesive contact and aggregation of epithelial cells in a Ca(2+)-independent manner. Also involved in striated muscle regeneration and repair and in the regulation of cell spreading. Belongs to the popeye family.

Protein type: Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 6q21

Cellular Component: tight junction; plasma membrane; integral to membrane; lateral plasma membrane

Molecular Function: cAMP binding; structural molecule activity

Biological Process: vesicle-mediated transport; regulation of cell shape; regulation of membrane potential; muscle development; positive regulation of locomotion; regulation of heart rate; hemopoietic progenitor cell differentiation; positive regulation of receptor recycling