Typical Testing Data/Standard Curve (for reference only)
Human Glucocorticoid Induced Tumor Necrosis Factor Receptor (GITR) ELISA Kit | GITR elisa kit
Human Glucocorticoid Induced Tumor Necrosis Factor Receptor (GITR) ELISA Kit
Gene Names
Tnfrsf18; AITR; Gitr
Reactivity
Human
Synonyms
Glucocorticoid Induced Tumor Necrosis Factor Receptor (GITR); Human Glucocorticoid Induced Tumor Necrosis Factor Receptor (GITR) ELISA Kit; TNFRSF18; AITR; Tumor Necrosis Factor Receptor Superfamily 18; Glucocorticoid-induced TNFR-related protein; Activation-inducible TNFR family receptor; GITR elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of GHSR. No significant cross-reactivity or interference between GHSR and analogues was observed.
Sequence Length
132
Samples
Rat Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates And Other Biological Fluids
Assay Type
Quantitative Sandwich
Detection Range
0.156-10ng/mL
Sensitivity
0.058ng/mL.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level GHSR were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level GHSR were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for GITR elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of GHSR in rat serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to GHSR. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to GHSR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain GHSR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of GHSR in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to GHSR. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to GHSR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain GHSR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of GHSR in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for GITR elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
14,106 Da
NCBI Official Full Name
glucocorticoid induced TNFR family related protein
NCBI Official Synonym Full Names
tumor necrosis factor receptor superfamily, member 18
NCBI Official Symbol
Tnfrsf18
NCBI Official Synonym Symbols
AITR; Gitr
NCBI Protein Information
tumor necrosis factor receptor superfamily member 18
UniProt Protein Name
Tumor necrosis factor receptor superfamily member 18
UniProt Gene Name
Tnfrsf18
UniProt Synonym Gene Names
Gitr
UniProt Entry Name
TNR18_MOUSE
Uniprot Description
TNFRSF18: Receptor for TNFSF18. Seems to be involved in interactions between activated T-lymphocytes and endothelial cells and in the regulation of T-cell receptor-mediated cell death. Mediated NF-kappa-B activation via the TRAF2/NIK pathway. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Receptor, cytokine; Apoptosis
Cellular Component: membrane; integral to plasma membrane; integral to membrane; plasma membrane; extracellular region; external side of plasma membrane
Molecular Function: protein binding
Biological Process: apoptosis
Research Articles on GITR
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Product Notes
The Human GITR tnfrsf18 (Catalog #AAA2021934) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2021934 ELISA Kit recognizes Human GITR. It is sometimes possible for the material contained within the vial of "Glucocorticoid Induced Tumor Necrosis Factor Receptor (GITR), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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