Rabbit SNTB2 Polyclonal Antibody | anti-SNTB2 antibody

SNTB2 Antibody

Cat. Num. AAA9612031

• Gene Names: SNTB2; SNT3; SNTL; SNT2B2; EST25263; D16S2531E
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(88%)
• Applications: Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: SNTB2; Polyclonal Antibody; SNTB2 Antibody; Syntrophin like; 59 kDa dystrophin associated protein A1 basic component 2; 59 kDa dystrophin-associated protein A1 basic component 2; Basic beta 2 syntrophin; Beta 2 syntrophin; Beta-2-syntrophin; D16S2531E; Dystrophin associated protein A1 59kD basic component 2; EST25263; SNT2B2; SNT3; Sntb2; SNTB2_HUMAN; SNTL; Syntrophin 3; Syntrophin beta 2 (dystrophin associated protein A1 59kDa basic component 2); Syntrophin-3; Syntrophin-like; anti-SNTB2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(88%)
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: SNTB2 Antibody detects endogenous levels of total SNTB2.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 540

• Applicable Applications for anti-SNTB2 antibody: Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; IF: 1:100-1:500; ICC: 1:100-1:500; IHC: 1:50-1:200; ELISA(peptide): 1:20,000-1:40,000
• Immunogen: A synthesized peptide derived from human SNTB2, corresponding to a region within the internal amino acids.
• Fragment: Fab fragment
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from B16F10 cells, using SNTB2 Antibody. The lane on the left was treated with blocking peptide.)

Immunohistochemistry (IHC)-Paraffin

(MBS9612031 at 1/100 staining Mouse heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

Immunofluorescence (IF)/Immunocytochemistry (ICC)

(MBS9612031 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(MBS9612031 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)

Related Product Information for anti-SNTB2 antibody

Function: Adapter protein that binds to and probably organizes the subcellular localization of a variety of membrane proteins. May link various receptors to the actin cytoskeleton and the dystrophin glycoprotein complex. May play a role in the regulation of secretory granules via its interaction with PTPRN.

Post Translational Modifications: Phosphorylated. Partially dephosphorylated upon insulin stimulation.

Subcellular Location: Membrane. Cytoplasmic vesicle>Secretory vesicle membrane>Peripheral membrane protein. Cell junction. Cytoplasm>Cytoskeleton. Note: Membrane-associated. In muscle, it is exclusively localized at the neuromuscular junction (By similarity). In insulinoma cell line, it is enriched in secretory granules.

Tissue Specificity: Ubiquitous. Isoform 1 is the predominant isoform. Weak level of isoform 2 is present in all tested tissues, except in liver and heart where it is highly expressed.

Subunit Structure: Monomer and homodimer (Probable). Interacts with the other members of the syntrophin family: SNTA1 and SNTB1; and with the sodium channel proteins SCN4A and SCN5A. Interacts with SAST, MAST205, microtubules and microtubule-associated proteins (By similarity). Interacts with the dystrophin protein DMD and related proteins DTNA and UTRN, and with the neuroregulin receptor ERBB4. Interacts with PTPRN when phosphorylated, protecting PTPRN from protein cleavage by CAPN1. Dephosphorylation upon insulin stimulation disrupts the interaction with PTPRN and results in the cleavage of PTPRN.

Similarity: The PH 1 domain mediates the oligomerization in a calcium dependent manner.The PDZ domain binds to the last three or four amino acids of ion channels and receptor proteins. The association with dystrophin or related proteins probably leaves the PDZ domain available to recruit proteins to the membrane (By similarity).The SU domain binds calmodulin in a calcium-dependent manner.Belongs to the syntrophin family.

NCBI and Uniprot Product Information

• NCBI GI #: 5803177
• NCBI GeneID: 6645
• NCBI Accession #: NP_006741
• NCBI GenBank Nucleotide #: NP_006741.1
• UniProt Accession #: Q13425
• Molecular Weight: Observed Molecular Weight: (Observed)57kD.; Predicted Molecular Weight: (Calculated)58kDa.
• NCBI Official Full Name: beta-2-syntrophin
• NCBI Official Synonym Full Names: syntrophin beta 2
• NCBI Official Symbol: SNTB2
• NCBI Official Synonym Symbols: SNT3; SNTL; SNT2B2; EST25263; D16S2531E
• NCBI Protein Information: beta-2-syntrophin
• UniProt Protein Name: Beta-2-syntrophin
• Protein Family: Beta-2-syntrophin
• UniProt Gene Name: SNTB2
• UniProt Synonym Gene Names: D16S2531E; SNT2B2; SNTL; SNT3; SNTL
• UniProt Entry Name: SNTB2_HUMAN

NCBI Description

Dystrophin is a large, rod-like cytoskeletal protein found at the inner surface of muscle fibers. Dystrophin is missing in Duchenne Muscular Dystrophy patients and is present in reduced amounts in Becker Muscular Dystrophy patients. The protein encoded by this gene is a peripheral membrane protein found associated with dystrophin and dystrophin-related proteins. This gene is a member of the syntrophin gene family, which contains at least two other structurally-related genes. [provided by RefSeq, Jul 2008]