Typical Testing Data/Standard Curve (for reference only)
(Human FSTL1 PicoKine ELISA Kit standard curve )
Human FSTL1 ELISA Kit | FSTL1 elisa kit
Human FSTL1 PicoKine ELISA Kit
Gene Names
FSTL1; FRP; FSL1; MIR198
Reactivity
Human
Synonyms
FSTL1; Human FSTL1 PicoKine ELISA Kit; Follistatin-related protein 1; follistatin-like 1; FLJ50214; FLJ52277; follistatin like 1; follistatin related protein; Follistatin-like protein 1; FRP; FSL1; FSTL1_HUMAN; Micro RNA 198; MIR198; miRNA198 included; FSTL1 elisa kit
Reactivity
Human
Specificity
Natural and recombinant human FSTL1
Sequence Length
308
Samples
Cell culture supernates, serum and plasma (heparin, EDTA)
Assay Type
Sandwich
Detection Range
312pg/ml-20,000pg/ml
Sensitivity
< 10pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Typical Testing Data/Standard Curve (for reference only)
(Human FSTL1 PicoKine ELISA Kit standard curve )
Typical Testing Data/Standard Curve (for reference only)
(Human FSTL1 PicoKine ELISA Kit standard curve )
Related Product Information for FSTL1 elisa kit
Principle of the assay: human FSTL1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for FSTL1 has been precoated onto 96-well plates. Standards(NSO, M1-I308) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for FSTL1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human FSTL1 amount of sample captured in plate.
Background: Follistatin-related protein 1, also called FSTL1 or FRP is a protein that in humans is encoded by the FSTL1 gene. FSTL1 gene is mapped to chromosome 3q13.33 based on an alignment of FSTL1 sequence with the genomic sequence (GRCh37). This gene encodes a protein with similarity to follistatin, an activin-binding protein. It contains an FS module, a follistatin-like sequence containing 10 conserved cysteine residues. This gene product is thought to be an autoantigen associated with rheumatoid arthritis. This gene may modulate the action of some growth factors on cell proliferation and differentiation. It was suggested that fibroblast-secreted FSTL1 acts on macrophages to increase TNF and IL1B production and IL6 activity.
Background: Follistatin-related protein 1, also called FSTL1 or FRP is a protein that in humans is encoded by the FSTL1 gene. FSTL1 gene is mapped to chromosome 3q13.33 based on an alignment of FSTL1 sequence with the genomic sequence (GRCh37). This gene encodes a protein with similarity to follistatin, an activin-binding protein. It contains an FS module, a follistatin-like sequence containing 10 conserved cysteine residues. This gene product is thought to be an autoantigen associated with rheumatoid arthritis. This gene may modulate the action of some growth factors on cell proliferation and differentiation. It was suggested that fibroblast-secreted FSTL1 acts on macrophages to increase TNF and IL1B production and IL6 activity.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
31,228 Da
NCBI Official Full Name
Follistatin-related protein 1
NCBI Official Synonym Full Names
follistatin-like 1
NCBI Official Symbol
FSTL1
NCBI Official Synonym Symbols
FRP; FSL1; MIR198
NCBI Protein Information
follistatin-related protein 1; follistatin-like protein 1
UniProt Protein Name
Follistatin-related protein 1
Protein Family
UniProt Gene Name
FSTL1
UniProt Synonym Gene Names
FRP
UniProt Entry Name
FSTL1_HUMAN
NCBI Description
This gene encodes a protein with similarity to follistatin, an activin-binding protein. It contains an FS module, a follistatin-like sequence containing 10 conserved cysteine residues. This gene product is thought to be an autoantigen associated with rheumatoid arthritis. [provided by RefSeq, Jul 2008]
Uniprot Description
FSTL1: May modulate the action of some growth factors on cell proliferation and differentiation. Binds heparin.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 3q13.33
Cellular Component: extracellular space; extracellular region
Molecular Function: heparin binding; protein binding; calcium ion binding
Biological Process: response to starvation; BMP signaling pathway
Research Articles on FSTL1
Similar Products
Product Notes
The Human FSTL1 fstl1 (Catalog #AAA177300) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA177300 ELISA Kit recognizes Human FSTL1. It is sometimes possible for the material contained within the vial of "FSTL1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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