Human Frizzled Homolog 10 (FZD10) ELISA Kit | FZD10 elisa kit

Human Frizzled Homolog 10 (FZD10) ELISA Kit

Cat. Num. AAA162997

• Gene Names: FZD10; Fz10; FzE7; CD350; FZ-10; hFz10
• Reactivity: Human
• Synonyms: Frizzled Homolog 10 (FZD10); Human Frizzled Homolog 10 (FZD10) ELISA Kit; FZD10 elisa kit

• Reactivity: Human
• Sequence Length: 581

• Samples: Serum, plasma, cell culture supernates, cell lysates, tissue homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 15ng/L - 3000ng/L
• Sensitivity: 8.14ng/L
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 2 to 8 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FZD10 elisa kit

Intended Uses: This sandwich kit is for the accurate quantitative detection of human Frizzled-10 (also known as FZD10) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human FZD10 antibody. FZD10 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human FZD10 Antibody is added and binds to FZD10 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated FZD10 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human FZD10. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

NCBI and Uniprot Product Information

• NCBI GI #: 6005762
• NCBI GeneID: 11211
• NCBI Accession #: NP_009128.1
• NCBI GenBank Nucleotide #: NM_007197.3
• UniProt Accession #: Q9ULW2
• Molecular Weight: 65,336 Da
• NCBI Official Full Name: frizzled-10
• NCBI Official Synonym Full Names: frizzled class receptor 10
• NCBI Official Symbol: FZD10
• NCBI Official Synonym Symbols: Fz10; FzE7; CD350; FZ-10; hFz10
• NCBI Protein Information: frizzled-10
• UniProt Protein Name: Frizzled-10
• Protein Family: Frizzled
• UniProt Gene Name: FZD10
• UniProt Synonym Gene Names: Fz-10; hFz10

NCBI Description

This gene is a member of the frizzled gene family. Members of this family encode 7-transmembrane domain proteins that are receptors for the Wingless type MMTV integration site family of signaling proteins. Most frizzled receptors are coupled to the beta-catenin canonical signaling pathway. Using array analysis, expression of this intronless gene is significantly up-regulated in two cases of primary colon cancer. [provided by RefSeq, Jul 2008]

Uniprot Description

Receptor for Wnt proteins. Functions in the canonical Wnt/beta-catenin signaling pathway (). The canonical Wnt/beta-catenin signaling pathway leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues (Probable).