Human Frizzled-9 (FZD9) ELISA Kit | FZD9 elisa kit

Human Frizzled-9 (FZD9) ELISA Kit

Cat. Num. AAA287651

• Gene Names: FZD9; FZD3; CD349
• Reactivity: Human
• Synonyms: Frizzled-9 (FZD9); Human Frizzled-9 (FZD9) ELISA Kit; CD349; FZD3; frizzled 9; FZD9 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human FZD9. No significant cross-reactivity or interference between Human FZD9 and analogues was observed.
• Sequence Length: 591

• Assay Type: Quantitive Sandwich
• Samples: Serum, Plasma, Other Biological Fluids
• Detection Wavelength: 450 nm
• Intra-assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
• Inter-assay Precision (Precision between assays): Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
• CV (%): = SD/meanX100
• Intra-Assay: CV
• Inter-Assay: CV
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for FZD9 elisa kit

Description: This assay employs a two-site sandwich ELISA to quantitate FZD9 in samples. An antibody specific for FZD9 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FZD9 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FZD9 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FZD9 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Overview: The FZD9 gene is located within the Williams syndrome common deletion region of chromosome 7, and heterozygous deletion of the FZD9 gene may contribute to the Williams syndrome phenotype. FZD9 is expressed predominantly in brain, testis, eye, skeletal muscle, and kidney. In mouse, the wg-homologous Wnt1 gene is involved in early development of a large domain of the central nervous system encompassing much of the midbrain and rostral metencephalon.The potential function of FZD9 in transmitting a Wnt protein signal in the human brain and other tissues suggested to Wang et al. (1997) that heterozygous deletion of the FZD9 gene could contribute to the Williams syndrome phenotype.

NCBI and Uniprot Product Information

• NCBI GI #: 4503835
• NCBI GeneID: 8326
• NCBI Accession #: NP_003499.1
• NCBI GenBank Nucleotide #: NM_003508.2
• UniProt Accession #: O00144
• Molecular Weight: 64,466 Da
• NCBI Official Full Name: frizzled-9
• NCBI Official Synonym Full Names: frizzled class receptor 9
• NCBI Official Symbol: FZD9
• NCBI Official Synonym Symbols: FZD3; CD349
• NCBI Protein Information: frizzled-9
• UniProt Protein Name: Frizzled-9
• Protein Family: Frizzled
• UniProt Gene Name: FZD9
• UniProt Synonym Gene Names: FZD3; Fz-9; hFz9

NCBI Description

Members of the 'frizzled' gene family encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The FZD9 gene is located within the Williams syndrome common deletion region of chromosome 7, and heterozygous deletion of the FZD9 gene may contribute to the Williams syndrome phenotype. FZD9 is expressed predominantly in brain, testis, eye, skeletal muscle, and kidney. [provided by RefSeq, Jul 2008]

Uniprot Description

Receptor for WNT2 that is coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes (). Plays a role in neuromuscular junction (NMJ) assembly by negatively regulating the clustering of acetylcholine receptors (AChR) through the beta-catenin canonical signaling pathway (). May play a role in neural progenitor cells (NPCs) viability through the beta-catenin canonical signaling pathway by negatively regulating cell cycle arrest leading to inhibition of neuron apoptotic process (PubMed:27509850). During hippocampal development, regulates neuroblast proliferation and apoptotic cell death. Controls bone formation through non canonical Wnt signaling mediated via ISG15. Positively regulates bone regeneration through non canonical Wnt signaling ().