Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using PRKG2 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 1s.)
Rabbit PRKG2 Polyclonal Antibody | anti-PRKG2 antibody
PRKG2 Rabbit pAb
IF: 1:50-1:200
IP: 1:50-1:200
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using PRKG2 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 1s.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using PRKG2 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 1s.)
Immunoprecipitation (IP)
(Immunoprecipitation analysis of 600ug extracts of Mouse lung using 3ug PRKG2 antibody . Western blot was performed from the immunoprecipitate using PRKG2 antibody at a dilition of 1:1000.)
Immunoprecipitation (IP)
(Immunoprecipitation analysis of 600ug extracts of Mouse lung using 3ug PRKG2 antibody . Western blot was performed from the immunoprecipitate using PRKG2 antibody at a dilition of 1:1000.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a protein that belongs to the serine/threonine protein kinase family of proteins. The encoded protein plays a role in the regulation of fluid balance in the intestine. A similar protein in mouse is thought to regulate differentiation and proliferation of cells in the colon. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]
Uniprot Description
PKG2: an AGC kinase of the PKG family. Type II cGMP-dependent protein kinase is expressed at high levels in brain, lung and intestinal mucosa.
Protein type: Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.12; Kinase, protein; Protein kinase, AGC; AGC group; PKG family
Chromosomal Location of Human Ortholog: 4q13.1-q21.1
Cellular Component: nuclear membrane; apical plasma membrane; cytosol
Molecular Function: protein homodimerization activity; cGMP-dependent protein kinase activity; cGMP binding; ATP binding; protein kinase activity
Biological Process: circadian regulation of gene expression; regulation of nitric-oxide synthase activity; signal transduction; blood coagulation; protein amino acid phosphorylation; nitric oxide metabolic process
Research Articles on PRKG2
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Product Notes
The PRKG2 prkg2 (Catalog #AAA9143435) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PRKG2 Rabbit pAb reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PRKG2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunoprecipitation (IP). WB: 1:500-1:2000 IF: 1:50-1:200 IP: 1:50-1:200. Researchers should empirically determine the suitability of the PRKG2 prkg2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PRKG2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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