Human Flavin Containing Monooxygenase 1 (FMO1) ELISA Kit | FMO1 elisa kit

Human Flavin Containing Monooxygenase 1 (FMO1) ELISA Kit

Cat. Num. AAA2705815

• Reactivity: Human
• Synonyms: Flavin Containing Monooxygenase 1 (FMO1); Human Flavin Containing Monooxygenase 1 (FMO1) ELISA Kit; Dimethylaniline oxidase 1; Fetal hepatic flavin-containing monooxygenase 1; FMO1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Flavin Containing Monooxygenase 1 (FMO1).; No significant cross-reactivity or interference between Flavin Containing Monooxygenase 1 (FMO1) and analogues was observed.
• Sequence Length: 469

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.115ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Flavin Containing Monooxygenase 1 (FMO1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Flavin Containing Monooxygenase 1 (FMO1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FMO1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Flavin Containing Monooxygenase 1 (FMO1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Flavin Containing Monooxygenase 1 (FMO1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Flavin Containing Monooxygenase 1 (FMO1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Flavin Containing Monooxygenase 1 (FMO1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 57790274
• NCBI GeneID: 2326
• UniProt Accession #: Q01740; Q5QPT2; Q9UJC2; A8K248; B7Z3P4
• Molecular Weight: 52,813 Da
• NCBI Official Full Name: flavin containing monooxygenase 1
• NCBI Official Synonym Full Names: flavin containing monooxygenase 1
• NCBI Official Symbol: FMO1
• NCBI Protein Information: dimethylaniline monooxygenase [N-oxide-forming] 1
• UniProt Protein Name: Dimethylaniline monooxygenase [N-oxide-forming] 1
• Protein Family: Thiol-specific monooxygenase
• UniProt Gene Name: FMO1
• UniProt Synonym Gene Names: FMO 1

NCBI Description

Metabolic N-oxidation of the diet-derived amino-trimethylamine (TMA) is mediated by flavin-containing monooxygenase and is subject to an inherited FMO3 polymorphism in man resulting in a small subpopulation with reduced TMA N-oxidation capacity resulting in fish odor syndrome Trimethylaminuria. Three forms of the enzyme, FMO1 found in fetal liver, FMO2 found in adult liver, and FMO3 are encoded by genes clustered in the 1q23-q25 region. Flavin-containing monooxygenases are NADPH-dependent flavoenzymes that catalyzes the oxidation of soft nucleophilic heteroatom centers in drugs, pesticides, and xenobiotics. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2013]

Uniprot Description

This protein is involved in the oxidative metabolism of a variety of xenobiotics such as drugs and pesticides. Form I catalyzes the N-oxygenation of secondary and tertiary amines.