Human Fibroblast Growth Factor 20 (FGF20) ELISA Kit | FGF20 elisa kit

Human Fibroblast Growth Factor 20 (FGF20) ELISA Kit

Cat. Num. AAA2098012

• Gene Names: FGF20; RHDA2; FGF-20
• Reactivity: Human
• Synonyms: Fibroblast Growth Factor 20 (FGF20); Human Fibroblast Growth Factor 20 (FGF20) ELISA Kit; FGF20 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Fibroblast Growth Factor 20 (FGF20).; No significant cross-reactivity or interference between Fibroblast Growth Factor 20 (FGF20) and analogues was observed.
• Sequence Length: 211

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids
• Detection Range: 7.8-500pg/mL
• Sensitivity: < 3.5pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibroblast Growth Factor 20 (FGF20) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibroblast Growth Factor 20 (FGF20) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FGF20 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fibroblast Growth Factor 20 (FGF20). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fibroblast Growth Factor 20 (FGF20). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fibroblast Growth Factor 20 (FGF20), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Fibroblast Growth Factor 20 (FGF20) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for FGF20 elisa kit

Cytokine

NCBI and Uniprot Product Information

• NCBI GI #: 9789947
• NCBI GeneID: 26281
• NCBI Accession #: NP_062825.1
• NCBI GenBank Nucleotide #: NM_019851.2
• UniProt Accession #: Q9NP95; B2RPH5
• Molecular Weight: 23,499 Da
• NCBI Official Full Name: fibroblast growth factor 20
• NCBI Official Synonym Full Names: fibroblast growth factor 20
• NCBI Official Symbol: FGF20
• NCBI Official Synonym Symbols: RHDA2; FGF-20
• NCBI Protein Information: fibroblast growth factor 20
• UniProt Protein Name: Fibroblast growth factor 20
• Protein Family: Fibroblast growth factor
• UniProt Gene Name: FGF20
• UniProt Synonym Gene Names: FGF-20

NCBI Description

The protein encoded by this gene is a member of the fibroblast growth factor family. The fibroblast growth factors possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This gene product is a secreted neurotrophic factor but lacks a typical signal peptide. It is expressed in normal brain, particularly the cerebellum, and may regulate central nervous system development and function. Homodimerization of this protein was shown to regulate its receptor binding activity and concentration gradient in the extracellular matrix. Genetic variations of this gene have been associated with Parkinson disease susceptibility. [provided by RefSeq, Oct 2009]

Uniprot Description

Neurotrophic factor that regulates central nervous development and function.