Human Fibroblast Growth Factor 12 (FGF12) ELISA Kit | FGF12 elisa kit

Human Fibroblast Growth Factor 12 (FGF12) ELISA Kit

Cat. Num. AAA2020281

• Gene Names: FGF12; FHF1; FGF12B
• Reactivity: Human
• Synonyms: Fibroblast Growth Factor 12 (FGF12); Human Fibroblast Growth Factor 12 (FGF12) ELISA Kit; FGF12B; FHF1; Fibroblast Growth Factor Homologous Factor 1; Myocyte-Activating Factor; FGF12 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Fibroblast Growth Factor 12 (FGF12).; No significant cross-reactivity or interference between Fibroblast Growth Factor 12 (FGF12) and analogues was observed.
• Sequence Length: 127

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids
• Detection Range: 15.6-1000pg/mL
• Sensitivity: < 5.6pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibroblast Growth Factor 12 (FGF12) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibroblast Growth Factor 12 (FGF12) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FGF12 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fibroblast Growth Factor 12 (FGF12). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fibroblast Growth Factor 12 (FGF12). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fibroblast Growth Factor 12 (FGF12), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Fibroblast Growth Factor 12 (FGF12) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for FGF12 elisa kit

Cytokine

NCBI and Uniprot Product Information

• NCBI GI #: 1749791
• NCBI GeneID: 2257
• UniProt Accession #: Q99517; P61328
• Molecular Weight: 14,478 Da
• NCBI Official Full Name: fibroblast growth factor 12, partial
• NCBI Official Synonym Full Names: fibroblast growth factor 12
• NCBI Official Symbol: FGF12
• NCBI Official Synonym Symbols: FHF1; FGF12B
• NCBI Protein Information: fibroblast growth factor 12
• UniProt Protein Name: Fibroblast growth factor
• Protein Family: Fibroblast growth factor
• UniProt Gene Name: FGF12
• UniProt Synonym Gene Names: FGF
• UniProt Entry Name: Q99517_HUMAN

NCBI Description

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth, and invasion. This growth factor lacks the N-terminal signal sequence present in most of the FGF family members, but it contains clusters of basic residues that have been demonstrated to act as a nuclear localization signal. When transfected into mammalian cells, this protein accumulated in the nucleus, but was not secreted. The specific function of this gene has not yet been determined. Two alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

Uniprot Description

FGF12: Probably involved in nervous system development and function. Belongs to the heparin-binding growth factors family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytokine; Cell development/differentiation

Chromosomal Location of Human Ortholog: 3q28

Cellular Component: extracellular space; nucleus

Molecular Function: heparin binding; growth factor activity; sodium channel regulator activity; fibroblast growth factor receptor binding

Biological Process: nervous system development; synaptic transmission; fibroblast growth factor receptor signaling pathway; cell-cell signaling; adult locomotory behavior; heart development; neuromuscular process; JNK cascade; signal transduction