Human FGF22 (Fibroblast growth factor 22) ELISA Kit | FGF22 elisa kit

Human FGF22 (Fibroblast growth factor 22) ELISA Kit

Cat. Num. AAA7608164

• Reactivity: Human
• Synonyms: FGF22 (Fibroblast growth factor 22); Human FGF22 (Fibroblast growth factor 22) ELISA Kit; FGF-22; Fibroblast growth factor 22; FGF22 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of FGF22. No significant cross-reactivity or interference between FGF22 and analogues was observed.

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 15.625-1000pg/ml
• Sensitivity: 9.375pg/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 2-8 degree C for est. ~6 months

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FGF22 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 10190672
• NCBI GeneID: 27006
• NCBI Accession #: NP_065688.1
• NCBI GenBank Nucleotide #: NM_020637.1
• UniProt Accession #: Q9HCT0; B2RPH4
• Molecular Weight: 19,663 Da
• NCBI Official Full Name: fibroblast growth factor 22
• NCBI Official Synonym Full Names: fibroblast growth factor 22
• NCBI Official Symbol: FGF22
• NCBI Protein Information: fibroblast growth factor 22; FGF-22
• UniProt Protein Name: Fibroblast growth factor 22
• Protein Family: Fibroblast growth factor
• UniProt Gene Name: FGF22
• UniProt Synonym Gene Names: FGF-22
• UniProt Entry Name: FGF22_HUMAN

NCBI Description

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. The mouse homolog of this gene was found to be preferentially expressed in the inner root sheath of the hair follicle, which suggested a role in hair development. [provided by RefSeq, Jul 2008]

Uniprot Description

FGF22: Plays a role in the fasting response, glucose homeostasis, lipolysis and lipogenesis. Can stimulate cell proliferation (in vitro). May be involved in hair development. Belongs to the heparin-binding growth factors family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: Golgi apparatus; extracellular space; cell surface; nucleolus; extracellular region

Molecular Function: growth factor activity; fibroblast growth factor receptor binding

Biological Process: epidermal growth factor receptor signaling pathway; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; insulin receptor signaling pathway; innate immune response; cell differentiation