Rabbit anti-Human FADS3 Polyclonal Antibody | anti-FADS3 antibody

FADS3, CT (FADS3, CYB5RP, Fatty acid desaturase 3, Cytochrome b5-related protein) (AP)

Cat. Num. AAA6297625

• Gene Names: FADS3; CYB5RP; LLCDL3
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: FADS3; Polyclonal Antibody; CT (FADS3; CYB5RP; Fatty acid desaturase 3; Cytochrome b5-related protein) (AP); Cytochrome b5-related protein; anti-FADS3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-FADS3 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: FADS3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 327-355 amino acids from the C-terminal region of human FADS3.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(FADS3 Antibody (C-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the FADS3 antibody detected the FADS3 protein (arrow).)

Related Product Information for anti-FADS3 antibody

The protein encoded by this gene is a member of the fatty acid desaturase (FADS) gene family. Desaturase enzymes regulate unsaturation of fatty acids through the introduction of double bonds between defined carbons of the fatty acyl chain. FADS family members are considered fusion products composed of an N-terminal cytochrome b5-like domain and a C-terminal multiple membrane-spanning desaturase portion, both of which are characterized by conserved histidine motifs. This gene is clustered with family members FADS1 and FADS2 at 11q12-q13.1; this cluster is thought to have arisen evolutionarily from gene duplication based on its similar exon/intron organization.

Product Categories/Family for anti-FADS3 antibody

Antibodies; Enzymes; Cytochrome

References

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Mathias, R.A., et al. J. Lipid Res. 51(9):2766-2774(2010). Barber, M.J., et al. PLoS ONE 5 (3), E9763 (2010): Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009). Hicks, A.A., et al. PLoS Genet. 5 (10), E1000672 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 13375616
• NCBI GeneID: 3995
• NCBI Accession #: NP_068373.1
• NCBI GenBank Nucleotide #: NM_021727.4
• UniProt Accession #: Q9Y5Q0; O60426
• Molecular Weight: 51,145 Da
• NCBI Official Full Name: fatty acid desaturase 3
• NCBI Official Synonym Full Names: fatty acid desaturase 3
• NCBI Official Symbol: FADS3
• NCBI Official Synonym Symbols: CYB5RP; LLCDL3
• NCBI Protein Information: fatty acid desaturase 3
• UniProt Protein Name: Fatty acid desaturase 3
• Protein Family: Fatty acid desaturase
• UniProt Gene Name: FADS3
• UniProt Synonym Gene Names: CYB5RP
• UniProt Entry Name: FADS3_HUMAN

NCBI Description

The protein encoded by this gene is a member of the fatty acid desaturase (FADS) gene family. Desaturase enzymes regulate unsaturation of fatty acids through the introduction of double bonds between defined carbons of the fatty acyl chain. FADS family members are considered fusion products composed of an N-terminal cytochrome b5-like domain and a C-terminal multiple membrane-spanning desaturase portion, both of which are characterized by conserved histidine motifs. This gene is clustered with family members FADS1 and FADS2 at 11q12-q13.1; this cluster is thought to have arisen evolutionarily from gene duplication based on its similar exon/intron organization. [provided by RefSeq, Jul 2008]

Uniprot Description

FADS3: is a member of the fatty acid desaturase (FADS) gene family. Desaturase enzymes regulate unsaturation of fatty acids through the introduction of double bonds between defined carbons of the fatty acyl chain. FADS family members are considered fusion products composed of an N-terminal cytochrome b5-like domain and a C-terminal multiple membrane-spanning desaturase portion, both of which are characterized by conserved histidine motifs. This gene is clustered with family members FADS1 and FADS2 at 11q12-q13.1; this cluster is thought to have arisen evolutionarily from gene duplication based on its similar exon/intron organization. [provided by RefSeq, Jul 2008]

Protein type: Membrane protein, multi-pass; EC 1.14.19.-; Membrane protein, integral; Oxidoreductase

Chromosomal Location of Human Ortholog: 11q12-q13.1

Cellular Component: endoplasmic reticulum membrane; membrane; integral to membrane

Molecular Function: iron ion binding; heme binding; oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water

Biological Process: unsaturated fatty acid biosynthetic process