Human Eukaryotic peptide chain release factor GTP-binding subunit ERF3A (GSPT1) ELISA Kit | GSPT1 elisa kit

Human Eukaryotic peptide chain release factor GTP-binding subunit ERF3A (GSPT1) ELISA Kit

Cat. Num. AAA7269064

• Reactivity: Human
• Synonyms: Eukaryotic peptide chain release factor GTP-binding subunit ERF3A (GSPT1); Human Eukaryotic peptide chain release factor GTP-binding subunit ERF3A (GSPT1) ELISA Kit; GSPT1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of GSPT1. No significant cross-reactivity or interference between GSPT1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GSPT1 and all the analogues, therefore, cross reaction may still exist in some cases.

• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Assay Type: Quantitative Competitive
• Sensitivity: 0.1 ng/mL
• Preparation and Storage: Store at 2-8 degree C.; The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GSPT1 elisa kit

Intended Uses: This GSPT1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human GSPT1. This ELISA kit for research use only, not for therapeutic or test applications!

Principle of the Assay: GSPT1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-GSPT1 antibody and an GSPT1-HRP conjugate. The assay sample and buffer are incubated together with GSPT1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GSPT1 concentration since GSPT1 from samples and GSPT1-HRP conjugate compete for the anti-GSPT1 antibody binding site. Since the number of sites is limited, as more sites are occupied by GSPT1 from the sample, fewer sites are left to bind GSPT1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GSPT1 concentration in each sample is interpolated from this standard curve.