Human Epiphycan (EPYC) ELISA Kit | EPYC elisa kit

Human Epiphycan (EPYC) ELISA Kit

Cat. Num. AAA450698

• Gene Names: EPYC; PGLB; DSPG3; Pg-Lb; SLRR3B
• Reactivity: Human
• Synonyms: Epiphycan (EPYC); Human Epiphycan (EPYC) ELISA Kit; DSPG3; Pg-Lb; SLRR3B; Dermatan Sulphate Proteoglycan 3; Dermatan Sulfate Proteoglycan 3; Proteoglycan-Lb; Small chondroitin/dermatan sulfate proteoglycan; EPYC elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of EPYC. No significant cross-reactivity or interference between EPYC and analogues was observed.
• Sequence Length: 322

• Samples: Tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.056ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level EPYC were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level EPYC were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for EPYC elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of EPYC in human tissue homogenates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to EPYC. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to EPYC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain EPYC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of EPYC in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 68800360
• NCBI GeneID: 1833
• NCBI Accession #: NP_004941.2
• NCBI GenBank Nucleotide #: NM_004950.4
• UniProt Accession #: Q99645; Q8NEJ5; A8K3M7
• Molecular Weight: 36,637 Da
• NCBI Official Full Name: epiphycan
• NCBI Official Synonym Full Names: epiphycan
• NCBI Official Symbol: EPYC
• NCBI Official Synonym Symbols: PGLB; DSPG3; Pg-Lb; SLRR3B
• NCBI Protein Information: epiphycan
• UniProt Protein Name: Epiphycan
• Protein Family: Epiphycan
• UniProt Gene Name: EPYC
• UniProt Synonym Gene Names: DSPG3; PGLB; SLRR3B; PG-Lb

NCBI Description

Dermatan sulfate proteoglycan 3 is a member of the small leucine-rich repeat proteoglycan family. This gene is composed of seven exons. It regulates fibrillogenesis by interacting with collagen fibrils and other extracellular matrix proteins. [provided by RefSeq, Jul 2008]

Uniprot Description

May have a role in bone formation and also in establishing the ordered structure of cartilage through matrix organization.