Netrin-1 Recombinant Protein | NTN1 recombinant protein

Netrin-1 (human), (recombinant)

Cat. Num. AAA566240

• Gene Names: NTN1; NTN1L
• Reactivity: Human, Mouse, Rat
• Applications: ELISA
• Purity: >=90% (SDS-PAGE); Purified by multi-step chromatography
• Synonyms: Netrin-1; Netrin-1 (human); (recombinant); NTN1 recombinant protein

• Host: HEK293 cells
• Reactivity: Human, Mouse, Rat
• Specificity: Tests positive for binding to human Unc5b by ELISA. Previous lots produced from the same cell line bind to human, mouse and rat receptors UNC5H and DCC
• Purity/Purification: >=90% (SDS-PAGE); Purified by multi-step chromatography
• Form/Format: Lyophilized. Contains PBS
• Concentration: 0.1mg/ml after reconstitution (varies by lot)

• Applicable Applications for NTN1 recombinant protein: ELISA (EIA)
• Source Note: The extracellular domain of human netrin-1 (aa 28-604) is fused at the N-terminus to a DYKDDDDK-tag
• Preparation and Storage: Stable for at least 6 months after receipt when stored at -20 degree C; Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20 degree C; Shipping: Shipped on Blue Ice

Related Product Information for NTN1 recombinant protein

The Netrins (whose name means "one who guides" in Sanskrit) are a family of laminin-related secreted protein. In mice, human and rat, netrin-1, netrin-3/NTL2, Netrin-4 and G-Netrins have been cloned. Netrin-1 is predominantly expressed by floor plate cells as commissural axons extend toward the ventral midline. Netrin-1 induces axonal outgrowth, axon orientation and neuronal migration in neuronal development. It is chemoattractive for some neurons and chemorepellent for others. To carry its functions, netrin-1 interacts with specific receptors: DCC/Neogenin and UNC-5. These receptors belong to the family of "dependence receptors" that induce apoptosis in the absence of their respective ligand, and promote survival when bound to their ligand. The Netrin-1 mediated survival signal inhibits p53-induced apoptosis. Netrin-1 is also expressed in organs such as pancreas, lung, bowel, bone and mammary gland. In these tissues, netrin-1 has different roles such as invasion of epithelial cells, leukocytes migration, cell adhesion or angiogenesis. Netrin-1, as a survival factor, also contributes to the control of tumorigenesis. Historical results have shown that netrin-1 induces axon outgrowth and inhibits DCC- and UNC5H2-mediated apoptosis in HEK 293 cells.

Product Categories/Family for NTN1 recombinant protein

Cellular Analysis; Cell Signaling

NCBI and Uniprot Product Information

• NCBI GI #: 148613884
• NCBI GeneID: 9423
• NCBI Accession #: NP_004813.2
• NCBI GenBank Nucleotide #: NM_004822.2
• UniProt Accession #: O95631; E9KL51
• Molecular Weight: 67,748 Da
• NCBI Official Full Name: netrin-1
• NCBI Official Synonym Full Names: netrin 1
• NCBI Official Symbol: NTN1
• NCBI Official Synonym Symbols: NTN1L
• NCBI Protein Information: netrin-1; netrin 1, mouse, homolog of; epididymis tissue protein Li 131P
• UniProt Protein Name: Netrin-1
• Protein Family: Netrin
• UniProt Gene Name: NTN1
• UniProt Synonym Gene Names: NTN1L
• UniProt Entry Name: NET1_HUMAN

NCBI Description

Netrin is included in a family of laminin-related secreted proteins. The function of this gene has not yet been defined; however, netrin is thought to be involved in axon guidance and cell migration during development. Mutations and loss of expression of netrin suggest that variation in netrin may be involved in cancer development. [provided by RefSeq, Jul 2008]

Uniprot Description

NTN1: Netrins control guidance of CNS commissural axons and peripheral motor axons. Its association with either DCC or some UNC5 receptors will lead to axon attraction or repulsion, respectively. It also serve as a survival factor via its association with its receptors which prevent the initiation of apoptosis. Involved in tumorigenesis by regulating apoptosis.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 17p13.1

Cellular Component: cytoplasm; extracellular region; basement membrane

Molecular Function: protein binding

Biological Process: axon guidance; cell-cell adhesion; inner ear morphogenesis; establishment of nucleus localization; apoptosis; positive regulation of axon extension; positive regulation of cell proliferation; neuron migration; negative regulation of axon extension; regulation of cell migration; anterior/posterior axon guidance