Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Epididymal-specific lipocalin-6 (LCN6) Standard Curve.)
Human Epididymal-Specific Lipocalin-6 (LCN6) ELISA Kit | LCN6 elisa kit
Human Epididymal-Specific Lipocalin-6 (LCN6) ELISA Kit
Gene Names
LCN6; LCN5; hLcn5; UNQ643
Reactivity
Human
Synonyms
Epididymal-Specific Lipocalin-6 (LCN6); Human Epididymal-Specific Lipocalin-6 (LCN6) ELISA Kit; LCN6; RP11-216L13.2; LCN5; UNQ643; hLcn5; epididymal-specific lipocalin LCN6; lipocalin 5; LCN6 elisa kit
Reactivity
Human
Specificity
Human Epididymal-specific lipocalin-6 (LCN6) ELISA Kit has high sensitivity and excellent specificity for detection of Human LCN6. No significant cross-reactivity or interference between Human LCN6 and analogues was observed.
Sequence Length
163
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Epididymal-specific lipocalin-6 (LCN6) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Epididymal-specific lipocalin-6 (LCN6) Standard Curve.)
Related Product Information for LCN6 elisa kit
Description: This Human Epididymal-specific lipocalin-6 (LCN6) ELISA Kit employs a two-site sandwich ELISA to quantitate LCN6 in samples. An antibody specific for LCN6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LCN6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LCN6 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LCN6 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Using database analysis and cDNA sequencing from a human caput library, Hamil et al. (2003) identified LCN6. The 163-amino acid LCN6 protein contains a predicted 20-amino acid signal peptide.human LCN6 is 93% identical to rhesus monkey Lcn6 and lacks a second, C-terminal cysteine found in many lipocalins. The LCN6 protein contains multiple phosphorylation sites, and structural prediction programs showed that LCN6 displays the characteristic lipocalin 3-dimensional structure. Northern blot analysis revealed robust expression of a 1-kb LCN6 transcript in epididymis. Using Immunohistochemistry, strong expression of LCN6 protein in the caput epithelium and lumen; Immunofluorescence of human spermatozoa showed strong LCN6 expression in large patches in the postacrosomal region of the head.
Background: Using database analysis and cDNA sequencing from a human caput library, Hamil et al. (2003) identified LCN6. The 163-amino acid LCN6 protein contains a predicted 20-amino acid signal peptide.human LCN6 is 93% identical to rhesus monkey Lcn6 and lacks a second, C-terminal cysteine found in many lipocalins. The LCN6 protein contains multiple phosphorylation sites, and structural prediction programs showed that LCN6 displays the characteristic lipocalin 3-dimensional structure. Northern blot analysis revealed robust expression of a 1-kb LCN6 transcript in epididymis. Using Immunohistochemistry, strong expression of LCN6 protein in the caput epithelium and lumen; Immunofluorescence of human spermatozoa showed strong LCN6 expression in large patches in the postacrosomal region of the head.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Epididymal-specific lipocalin-6
NCBI Official Synonym Full Names
lipocalin 6
NCBI Official Symbol
LCN6
NCBI Official Synonym Symbols
LCN5; hLcn5; UNQ643
NCBI Protein Information
epididymal-specific lipocalin-6
UniProt Protein Name
Epididymal-specific lipocalin-6
Protein Family
UniProt Gene Name
LCN6
UniProt Synonym Gene Names
LCN5
UniProt Entry Name
LCN6_HUMAN
Uniprot Description
LCN6: May play a role in male fertility. Belongs to the calycin superfamily. Lipocalin family.
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 9q34.3
Cellular Component: extracellular region
Biological Process: single fertilization
Research Articles on LCN6
Similar Products
Product Notes
The Human LCN6 lcn6 (Catalog #AAA9716039) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9716039 ELISA Kit recognizes Human LCN6. It is sometimes possible for the material contained within the vial of "Epididymal-Specific Lipocalin-6 (LCN6), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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