Human Ephrin-B2 (EFNB2) ELISA Kit | EFNB2 elisa kit

Human Ephrin-B2 (EFNB2) ELISA Kit

Cat. Num. AAA2800224

• Gene Names: EFNB2; HTKL; EPLG5; Htk-L; LERK5
• Reactivity: Human
• Synonyms: Ephrin-B2 (EFNB2); Human Ephrin-B2 (EFNB2) ELISA Kit; RP11-272L14.1; EPLG5; HTKL; Htk-L; LERK5; MGC126226; MGC126227; MGC126228; HTK ligand; eph-related receptor tyrosine kinase ligand 5; ephrin B2; ligand of eph-related kinase 5; EFNB2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human EFNB2. No significant cross-reactivity or interference between Human EFNB2 and analogues was observed.
• Sequence Length: 333

• Assay Type: Quantitive Sandwich
• Samples: Serum, Plasma, Other Biological Fluids
• Detection Wavelength: 450 nm
• Intra-assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
• Inter-assay Precision (Precision between assays): Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
• CV (%): = SD/meanX100
• Intra-Assay: CV
• Inter-Assay: CV
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for EFNB2 elisa kit

This assay employs a two-site sandwich ELISA to quantitate EFNB2 in samples. An antibody specific for EFNB2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any EFNB2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for EFNB2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EFNB2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 4758250
• NCBI GeneID: 1948
• NCBI Accession #: NP_004084.1
• NCBI GenBank Nucleotide #: NM_004093.3
• UniProt Accession #: P52799; Q5JV56
• Molecular Weight: 36,923 Da
• NCBI Official Full Name: ephrin-B2
• NCBI Official Synonym Full Names: ephrin B2
• NCBI Official Symbol: EFNB2
• NCBI Official Synonym Symbols: HTKL; EPLG5; Htk-L; LERK5
• NCBI Protein Information: ephrin-B2
• UniProt Protein Name: Ephrin-B2
• Protein Family: Ephrin
• UniProt Gene Name: EFNB2
• UniProt Synonym Gene Names: EPLG5; HTKL; LERK5; LERK-5; HTK-L

NCBI Description

This gene encodes a member of the ephrin (EPH) family. The ephrins and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, especially in the nervous system and in erythropoiesis. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. This gene encodes an EFNB class ephrin which binds to the EPHB4 and EPHA3 receptors. [provided by RefSeq, Jul 2008]

Uniprot Description

Cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Binds to receptor tyrosine kinase including EPHA4, EPHA3 and EPHB4. Together with EPHB4 plays a central role in heart morphogenesis and angiogenesis through regulation of cell adhesion and cell migration. EPHB4-mediated forward signaling controls cellular repulsion and segregation from EFNB2-expressing cells. May play a role in constraining the orientation of longitudinally projecting axons.