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Typical Testing Data/Standard Curve (for reference only) (Fig.1. Human Ephrin-B2 (EFNB2) Standard Curve.)

Human Ephrin-B2 (EFNB2) ELISA Kit | EFNB2 elisa kit

Human Ephrin-B2 (EFNB2) ELISA Kit

Gene Names
EFNB2; HTKL; EPLG5; Htk-L; LERK5
Reactivity
Human
Synonyms
Ephrin-B2 (EFNB2); Human Ephrin-B2 (EFNB2) ELISA Kit; EFNB2; RP11-272L14.1; EPLG5; HTKL; Htk-L; LERK5; MGC126226; MGC126227; MGC126228; HTK ligand; eph-related receptor tyrosine kinase ligand 5; ephrin B2; ligand of eph-related kinase 5; EFNB2 elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Specificity
Human Ephrin-B2 (EFNB2) ELISA Kit has high sensitivity and excellent specificity for detection of Human EFNB2. No significant cross-reactivity or interference between Human EFNB2 and analogues was observed.
Sequence Length
333
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Human Ephrin-B2 (EFNB2) Standard Curve.)

Typical Testing Data/Standard Curve (for reference only) (Fig.1. Human Ephrin-B2 (EFNB2) Standard Curve.)
Related Product Information for EFNB2 elisa kit
Description: This Human Ephrin-B2 (EFNB2) ELISA Kit employs a two-site sandwich ELISA to quantitate EFNB2 in samples. An antibody specific for EFNB2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any EFNB2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for EFNB2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EFNB2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: Ephrin-B2 is a member of the ephrin (EPH) family. The ephrins and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, especially in the nervous system and in erythropoiesis. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. This gene encodes an EFNB class ephrin which binds to the EPHB4 and EPHA3 receptors.Binds to the receptor tyrosine kinases EPHB4 and EPHA3. May play a role in constraining the orientation of longitudinally projecting axons.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
ephrin-B2
NCBI Official Synonym Full Names
ephrin B2
NCBI Official Symbol
EFNB2
NCBI Official Synonym Symbols
HTKL; EPLG5; Htk-L; LERK5
NCBI Protein Information
ephrin-B2
UniProt Protein Name
Ephrin-B2
Protein Family
UniProt Gene Name
EFNB2
UniProt Synonym Gene Names
EPLG5; HTKL; LERK5; LERK-5; HTK-L
UniProt Entry Name
EFNB2_HUMAN

NCBI Description

This gene encodes a member of the ephrin (EPH) family. The ephrins and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, especially in the nervous system and in erythropoiesis. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. This gene encodes an EFNB class ephrin which binds to the EPHB4 and EPHA3 receptors. [provided by RefSeq, Jul 2008]

Research Articles on EFNB2

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Product Notes

The Human EFNB2 efnb2 (Catalog #AAA9716098) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9716098 ELISA Kit recognizes Human EFNB2. It is sometimes possible for the material contained within the vial of "Ephrin-B2 (EFNB2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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