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Western Blot (WB) (Western blotAll lanes: ENO2antibody at 1.5ug/mlLane 1: Mouse heart tissueLane 2: Mouse liver tissueLane 3: Mouse brain tissueLane 4: Mouse kidney tissueLane 5: A549 whole cell lysateLane 6: Jurkat whole cell lysateSecondaryGoat polyclonal to Rabbit IgG at 1/10000 dilutionPredicted band size: 48,43 kDaObserved band size: 47 kDa)

Rabbit anti-Human Gamma-enolase Polyclonal Antibody | anti-ENO2 antibody

Rabbit anti-human Gamma-enolase polyclonal Antibody

Gene Names
ENO2; NSE; HEL-S-279
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Caprylic Acid Ammonium Sulfate Precipitation Purified
Synonyms
Gamma-enolase; Polyclonal Antibody; Rabbit anti-human Gamma-enolase polyclonal Antibody; 2-phospho-D-glycerate hydro-lyase Enolase 2 Neural enolase Neuron-specific enolase NSE; anti-ENO2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Caprylic Acid Ammonium Sulfate Precipitation Purified
Sequence Length
434
Applicable Applications for anti-ENO2 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Storage Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Conjugate
Non-conjugated
Immunogen
Recombinant human Gamma-enolase protein
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-31860 / sc-31859
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western blotAll lanes: ENO2antibody at 1.5ug/mlLane 1: Mouse heart tissueLane 2: Mouse liver tissueLane 3: Mouse brain tissueLane 4: Mouse kidney tissueLane 5: A549 whole cell lysateLane 6: Jurkat whole cell lysateSecondaryGoat polyclonal to Rabbit IgG at 1/10000 dilutionPredicted band size: 48,43 kDaObserved band size: 47 kDa)

Western Blot (WB) (Western blotAll lanes: ENO2antibody at 1.5ug/mlLane 1: Mouse heart tissueLane 2: Mouse liver tissueLane 3: Mouse brain tissueLane 4: Mouse kidney tissueLane 5: A549 whole cell lysateLane 6: Jurkat whole cell lysateSecondaryGoat polyclonal to Rabbit IgG at 1/10000 dilutionPredicted band size: 48,43 kDaObserved band size: 47 kDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded mouse brain using MBS715783 at dilution of 1:50)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded mouse brain using MBS715783 at dilution of 1:50)
Related Product Information for anti-ENO2 antibody
Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
42,707 Da
NCBI Official Full Name
gamma-enolase
NCBI Official Synonym Full Names
enolase 2 (gamma, neuronal)
NCBI Official Symbol
ENO2
NCBI Official Synonym Symbols
NSE; HEL-S-279
NCBI Protein Information
gamma-enolase
UniProt Protein Name
Gamma-enolase
Protein Family
UniProt Gene Name
ENO2
UniProt Synonym Gene Names
NSE
UniProt Entry Name
ENOG_HUMAN

NCBI Description

This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates. [provided by RefSeq, Jul 2008]

Uniprot Description

ENO2: an enzyme with 2-phospho-D-glycerate hydro-lyase activity. One of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates.

Protein type: Carbohydrate Metabolism - glycolysis and gluconeogenesis; EC 4.2.1.11; Lyase

Chromosomal Location of Human Ortholog: 12p13

Cellular Component: cytosol; extracellular space; myelin sheath; perikaryon; phosphopyruvate hydratase complex; photoreceptor inner segment; plasma membrane

Molecular Function: magnesium ion binding; phosphopyruvate hydratase activity; protein binding

Biological Process: carbohydrate metabolic process; gluconeogenesis; glucose metabolic process

Research Articles on ENO2

Similar Products

Product Notes

The ENO2 eno2 (Catalog #AAA715783) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-human Gamma-enolase polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Gamma-enolase can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Researchers should empirically determine the suitability of the ENO2 eno2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Gamma-enolase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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