Human Endothelin Receptor B (ETRB) ELISA Kit | ETRB elisa kit

Human Endothelin Receptor B (ETRB) ELISA Kit

Cat. Num. AAA450159

• Gene Names: EDNRB; ETB; ET-B; ETB1; ETBR; ETRB; HSCR; WS4A; ABCDS; ET-BR; HSCR2
• Reactivity: Human
• Synonyms: Endothelin Receptor B (ETRB); Human Endothelin Receptor B (ETRB) ELISA Kit; ATP receptor; P2X purinoceptor 7; P2X7; P2Z receptor; Purinergic receptor; ETRB elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of ETRB. No significant cross-reactivity or interference between ETRB and analogues was observed.
• Sequence Length: 442

• Samples: Tissue homogenates, cell lysates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.312-20ng/mL
• Sensitivity: 0.113ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ETRB were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ETRB were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ETRB elisa kit

Intended Uses: This kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of ETRB in human tissue homogenates, cell lysates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ETRB. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to ETRB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ETRB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of ETRB in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4557547
• NCBI GeneID: 1910
• NCBI Accession #: NP_000106.1
• NCBI GenBank Nucleotide #: NM_000115.4
• UniProt Accession #: P24530; O15343; Q59GB1; Q5W0G9; Q8NHM6; Q8NHM7; Q8NHM8; Q8NHM9; Q9UD23; Q9UQK3; A2A2Z8; A8K3T4; AAB24922
• Molecular Weight: 59,480 Da
• NCBI Official Full Name: endothelin B receptor isoform 1
• NCBI Official Synonym Full Names: endothelin receptor type B
• NCBI Official Symbol: EDNRB
• NCBI Official Synonym Symbols: ETB; ET-B; ETB1; ETBR; ETRB; HSCR; WS4A; ABCDS; ET-BR; HSCR2
• NCBI Protein Information: endothelin B receptor
• UniProt Protein Name: Endothelin B receptor
• UniProt Gene Name: EDNRB
• UniProt Synonym Gene Names: ETRB; ET-B; ET-BR

NCBI Description

The protein encoded by this gene is a G protein-coupled receptor which activates a phosphatidylinositol-calcium second messenger system. Its ligand, endothelin, consists of a family of three potent vasoactive peptides: ET1, ET2, and ET3. Studies suggest that the multigenic disorder, Hirschsprung disease type 2, is due to mutations in the endothelin receptor type B gene. Alternative splicing and the use of alternative promoters results in multiple transcript variants. [provided by RefSeq, Oct 2016]

Uniprot Description

Non-specific receptor for endothelin 1, 2, and 3. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.