Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Elafin (PI3) Standard Curve.)
Human Elafin (PI3) ELISA Kit | PI3 elisa kit
Human Elafin (PI3) ELISA Kit
Gene Names
PI3; ESI; WAP3; SKALP; WFDC14; cementoin
Reactivity
Human
Synonyms
Elafin (PI3); Human Elafin (PI3) ELISA Kit; PI3; ESI; MGC13613; SKALP; WAP3; WFDC14; cementoin; WAP four-disulfide core domain 14; elafin; elastase-specific inhibitor; pre-elafin; protease inhibitor 3; skin-derived (SKALP); skin-derived antileukoprotei; PI3 elisa kit
Reactivity
Human
Specificity
Human Elafin (PI3) ELISA Kit has high sensitivity and excellent specificity for detection of Human PI3. No significant cross-reactivity or interference between Human PI3 and analogues was observed.
Sequence Length
117
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Elafin (PI3) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Elafin (PI3) Standard Curve.)
Related Product Information for PI3 elisa kit
Description: This Human Elafin (PI3) ELISA Kit employs a two-site sandwich ELISA to quantitate PI3 in samples. An antibody specific for PI3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PI3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PI3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PI3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Elafin is an elastase-specific inhibitor, which contains a WAP-type four-disulfide core (WFDC) domain, and is thus a member of the WFDC domain family. Chang et al. and Schalkwijk et al. described a low molecular weight elastase inhibitor derived from psoriatic skin and termed SKALP for 'skin-derived antileukoproteinase.' They demonstrated that this inhibitor can be found in the epidermis of several inflammatory skin diseases, but not in normal human epidermis. Wiedow et al. referred to this molecule as elafin, and Sallenave and Ryle referred to it as elastase-specific inhibitor. It is found in the urine of psoriatic patients, and immunohistochemical studies showed that SKALP/elafin is found in the suprabasal differentiated keratinocytes of psoriatic epidermis. Molhuizen et al. isolated a precursor of SKALP/elafin from cultured keratinocytes and cloned its cDNA.
Background: Elafin is an elastase-specific inhibitor, which contains a WAP-type four-disulfide core (WFDC) domain, and is thus a member of the WFDC domain family. Chang et al. and Schalkwijk et al. described a low molecular weight elastase inhibitor derived from psoriatic skin and termed SKALP for 'skin-derived antileukoproteinase.' They demonstrated that this inhibitor can be found in the epidermis of several inflammatory skin diseases, but not in normal human epidermis. Wiedow et al. referred to this molecule as elafin, and Sallenave and Ryle referred to it as elastase-specific inhibitor. It is found in the urine of psoriatic patients, and immunohistochemical studies showed that SKALP/elafin is found in the suprabasal differentiated keratinocytes of psoriatic epidermis. Molhuizen et al. isolated a precursor of SKALP/elafin from cultured keratinocytes and cloned its cDNA.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
elafin preproprotein
NCBI Official Synonym Full Names
peptidase inhibitor 3
NCBI Official Symbol
PI3
NCBI Official Synonym Symbols
ESI; WAP3; SKALP; WFDC14; cementoin
NCBI Protein Information
elafin
UniProt Protein Name
Elafin
Protein Family
UniProt Gene Name
PI3
UniProt Synonym Gene Names
WAP3; WFDC14; ESI; PI-3; SKALP
UniProt Entry Name
ELAF_HUMAN
NCBI Description
This gene encodes an elastase-specific inhibitor that functions as an antimicrobial peptide against Gram-positive and Gram-negative bacteria, and fungal pathogens. The protein contains a WAP-type four-disulfide core (WFDC) domain, and is thus a member of the WFDC domain family. Most WFDC gene members are localized to chromosome 20q12-q13 in two clusters: centromeric and telomeric. This gene belongs to the centromeric cluster. Expression of this gene is upgulated by bacterial lipopolysaccharides and cytokines. [provided by RefSeq, Oct 2014]
Uniprot Description
elafin: Neutrophil and pancreatic elastase-specific inhibitor of skin. It may prevent elastase-mediated tissue proteolysis.
Protein type: Extracellular matrix; Inhibitor; Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 20q13.12
Cellular Component: proteinaceous extracellular matrix
Molecular Function: endopeptidase inhibitor activity
Research Articles on PI3
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Product Notes
The Human PI3 pi3 (Catalog #AAA9714883) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714883 ELISA Kit recognizes Human PI3. It is sometimes possible for the material contained within the vial of "Elafin (PI3), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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