Human EGF ELISA Kit | EGF elisa kit

Human EGF ELISA KIT

Cat. Num. AAA9424819

• Reactivity: Human
• Synonyms: EGF; Human EGF ELISA KIT; Human EGF; EGF elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant Human EGF Ligand

• Assay Type: Solid Phase Sandwich ELISA
• Samples: Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
• Detection Range: 31.25-2000pg/mL
• Sensitivity: 15pg/mL
• Assay Time: 3 hours
• Sample Volume: 20 uL
• Detection Method: Colorimetric
• Preparation and Storage: Store at 4 degree C

Typical Testing Data/Standard Curve (for reference only)

(Representative standard curve for EGF ELISA. EGF was diluted in serial two-fold steps in Sample Diluent.)

Related Product Information for EGF elisa kit

Epidermal growth factor (EGF) is the founding member of the EGF family that also includes TGFa, amphiregulin (AR), betacellulin (BTC), epiregulin (EPR), heparinbinding EGF-like growth factor (HB-EGF), epigen, and the neuregulins (NRG)1 through-6 (1). Members of the EGF family share a structural motif, the EGF-like domain, which is characterized by three intramolecular disulfide bonds that are formed by six similarly spaced conserved cysteine residues (2). All EGF family members are synthesized as type I transmembrane precursor proteins that may contain several EGF domains in the extracellular region. The 1207 amino acid (aa) human EGF precursor contains nine EGF domains and nine LDLR class B repeats. The mature protein consists of 53 aa and is generated by proteolytic excision of the EGF domain proximal to the transmembrane region (3). EGF is present in various body fluids, including blood, milk, urine, saliva, seminal fluid, pancreatic juice, cerebrospinal fluid, and amniotic fluid (4). Four ErbB (HER) family receptor tyrosine kinases including EGFR/ErbB1, ErbB2, ErbB3 and ErbB4, mediate responses to EGF family members (5). These receptors undergo a complex pattern of ligand induced homoor heterodimerization to transducer EGF family signals (6, 7). EGF binds ErbB1 and depending on the context, induces the formation of homodimers or heterodimers containing ErbB2. Dimerization results in autophosphorylation of the receptor at specific tyrosine residues to create docking sites for a variety of signaling molecules (5, 8). Biological activities ascribed to EGF include epithelial development, angiogenesis, inhibition of gastric acid secretion, fibroblast proliferation, and colony formation of epidermal cells in culture.

NCBI and Uniprot Product Information

• NCBI GI #: 958885663
• Molecular Weight: 18,895 Da
• NCBI Official Full Name: EGF, partial
• UniProt Protein Name: EGF
• Protein Family: EGF-like domain containing protein
• UniProt Gene Name: EGF