Human IL-15 ELISA Kit | IL-15 elisa kit

Human IL-15 ELISA KIT

Cat. Num. AAA9424823

• Gene Names: IL15; IL-15
• Reactivity: Human
• Synonyms: IL-15; Human IL-15 ELISA KIT; Human IL-15; IL-15 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant Human IL-15 Ligand

• Assay Type: Solid Phase Sandwich ELISA
• Samples: Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
• Detection Range: 7.8-500pg/mL
• Sensitivity: 4pg/mL
• Assay Time: 3 hours
• Sample Volume: 20 uL
• Detection Method: Colorimetric
• Preparation and Storage: Store at 4 degree C

Typical Testing Data/Standard Curve (for reference only)

(Representative standard curve for IL-15 ELISA. IL-15 was diluted in serial two-fold steps in Sample Diluent.)

Related Product Information for IL-15 elisa kit

Interleukin 15 (IL-15) is a 14 kDa cytokine that is structurally and functionally related to IL- 2 (1-3). Mature human IL-15 shares 70% amino acid sequence identity with mouse and rat IL-15. Alternate splicing generates isoforms of IL-15 with either a long or short signal peptide (LSP or SSP), and the SSP isoform is retained intracellularly (4). IL-15 associates with IL-15 Ra in the endoplasmic reticulum, and this complex is expressed on the cell surface (5, 6). The dominant mechanism of IL-15 action is known as transpresentation in which IL-15 and IL-15 Ra are coordinately expressed on the surface of one cell and interact with complexes of IL-2 Rb/gc on adjacent cells (7). This enables cells to respond to IL-15 even if they do not express IL-15 Ra (6, 8). Consistent with its shared use of IL-2 receptor subunits, IL-15 induces IL-2-like effects in lymphocyte development and homeostasis (3). It is particularly important for the maintenance and activation of NK cells and CD8+ memory T cells (3). IL-15 also exerts pleiotropic effects on other hematopoietic cells and nonimmune cells (2). Ligation of membraneassociated IL-15/IL-15Ra complexes induces reverse signaling that promotes cellular adhesion, tyrosine phosphorylation of intracellular proteins, and cytokine secretion by the IL-15/IL-15Ra expressing cells (9, 10).

NCBI and Uniprot Product Information

• NCBI GI #: 56181481
• NCBI GeneID: 397683
• Molecular Weight: 18,437 Da
• NCBI Official Full Name: IL-15, partial
• NCBI Official Symbol: IL15
• NCBI Official Synonym Symbols: IL-15
• NCBI Protein Information: interleukin-15
• UniProt Protein Name: Interleukin-15
• UniProt Gene Name: IL15
• UniProt Synonym Gene Names: IL-15

Uniprot Description

Cytokine that stimulates the proliferation of T-lymphocytes. Stimulation by IL-15 requires interaction of IL-15 with components of IL-2R, including IL-2R beta and probably IL-2R gamma but not IL-2R alpha ().