Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human EGF-like repeat and discoidin I-like domain-containing protein 3 (EDIL3) Standard Curve.)
Human EGF-Like Repeat And Discoidin I-Like Domain-Containing Protein 3 (EDIL3) ELISA Kit | EDIL3 elisa kit
Human EGF-Like Repeat And Discoidin I-Like Domain-Containing Protein 3 (EDIL3) ELISA Kit
Gene Names
EDIL3; DEL1
Reactivity
Human
Synonyms
EGF-Like Repeat And Discoidin I-Like Domain-Containing Protein 3 (EDIL3); Human EGF-Like Repeat And Discoidin I-Like Domain-Containing Protein 3 (EDIL3) ELISA Kit; EDIL3; DEL1; MGC26287; EGF-like repeats and discoidin I-like domains-containing protein 3; developmental endothelial locus-1; EDIL3 elisa kit
Reactivity
Human
Specificity
Human EGF-like repeat and discoidin I-like domain-containing protein 3 (EDIL3) ELISA Kit has high sensitivity and excellent specificity for detection of Human EDIL3. No significant cross-reactivity or interference between Human EDIL3 and analogues was observed.
Sequence Length
470
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human EGF-like repeat and discoidin I-like domain-containing protein 3 (EDIL3) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human EGF-like repeat and discoidin I-like domain-containing protein 3 (EDIL3) Standard Curve.)
Related Product Information for EDIL3 elisa kit
Description: This Human EGF-like repeat and discoidin I-like domain-containing protein 3 (EDIL3) ELISA Kit employs a two-site sandwich ELISA to quantitate EDIL3 in samples. An antibody specific for EDIL3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any EDIL3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for EDIL3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EDIL3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: EDIL3 is a glycoprotein that is secreted by endothelial cells and can associate with the endothelial cell surface and the extracellular matrix. EDIL3 is regulated by hypoxia or vascular injury and has been implicated in vascular remodeling during angiogenesis.The predicted 480-amino acid mouse and human proteins, which are 95% identical, contain a signal peptide, 3 epidermal growth factor (EGF)-like repeats, and 2 discoidin I-like repeats. Northern blot analysis revealed expression of a 6.5-kb transcript in highly vascularized mouse embryonic day-15.5 tissue and in mouse and human endothelial cells, but not in other adult tissues. In situ hybridization analysis showed Del1 expression in developing endocardium at 9.5 days postcoitus and in renal artery and hypertrophic chondrocytes at 13.5 days postcoitus.
Background: EDIL3 is a glycoprotein that is secreted by endothelial cells and can associate with the endothelial cell surface and the extracellular matrix. EDIL3 is regulated by hypoxia or vascular injury and has been implicated in vascular remodeling during angiogenesis.The predicted 480-amino acid mouse and human proteins, which are 95% identical, contain a signal peptide, 3 epidermal growth factor (EGF)-like repeats, and 2 discoidin I-like repeats. Northern blot analysis revealed expression of a 6.5-kb transcript in highly vascularized mouse embryonic day-15.5 tissue and in mouse and human endothelial cells, but not in other adult tissues. In situ hybridization analysis showed Del1 expression in developing endocardium at 9.5 days postcoitus and in renal artery and hypertrophic chondrocytes at 13.5 days postcoitus.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
EGF-like repeat and discoidin I-like domain-containing protein 3 isoform 2
NCBI Official Synonym Full Names
EGF like repeats and discoidin domains 3
NCBI Official Symbol
EDIL3
NCBI Official Synonym Symbols
DEL1
NCBI Protein Information
EGF-like repeat and discoidin I-like domain-containing protein 3
UniProt Protein Name
EGF-like repeat and discoidin I-like domain-containing protein 3
UniProt Gene Name
EDIL3
UniProt Synonym Gene Names
DEL1
UniProt Entry Name
EDIL3_HUMAN
NCBI Description
The protein encoded by this gene is an integrin ligand. It plays an important role in mediating angiogenesis and may be important in vessel wall remodeling and development. It also influences endothelial cell behavior. [provided by RefSeq, Jul 2008]
Uniprot Description
EDIL3: Promotes adhesion of endothelial cells through interaction with the alpha-v/beta-3 integrin receptor. Inhibits formation of vascular-like structures. May be involved in regulation of vascular morphogenesis of remodeling in embryonic development. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted; Cell adhesion; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 5q14
Research Articles on EDIL3
Similar Products
Product Notes
The Human EDIL3 edil3 (Catalog #AAA9716065) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9716065 ELISA Kit recognizes Human EDIL3. It is sometimes possible for the material contained within the vial of "EGF-Like Repeat And Discoidin I-Like Domain-Containing Protein 3 (EDIL3), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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